Recombinant Nymphaea alba ATP synthase subunit b, chloroplastic (atpF)
Description
Introduction to Recombinant Nymphaea alba ATP Synthase Subunit b, Chloroplastic (atpF)
Recombinant Nymphaea alba ATP synthase subunit b, chloroplastic (atpF), is a genetically engineered protein derived from the water lily species Nymphaea alba. This protein is part of the chloroplast ATP synthase complex, which plays a crucial role in photosynthesis by generating ATP from ADP using the proton gradient across the thylakoid membrane . The recombinant form of this protein is produced through biotechnological methods, typically in bacterial systems like E. coli, to facilitate research and potential applications in biotechnology and plant biology.
Function and Importance
ATP synthase is essential for photosynthesis and plant growth, as it converts light energy into chemical energy in the form of ATP . The chloroplastic ATP synthase subunit b (atpF) is one of the six subunits encoded by the chloroplast genome, along with atpA, atpE, atpH, and atpI . These subunits work together to form the complete enzyme complex that drives ATP synthesis.
Table 1: Overview of Chloroplast ATP Synthase Subunits
| Subunit | Function |
|---|---|
| atpA | Catalytic subunit |
| atpB | Catalytic subunit |
| atpE | Stalk subunit |
| atpF | Stalk subunit |
| atpH | Stalk subunit |
| atpI | Regulatory subunit |
Production and Characterization
Recombinant proteins like the Nymphaea alba ATP synthase subunit b are typically produced in bacterial systems. The production involves cloning the gene encoding the protein into an expression vector, followed by transformation into a host organism such as E. coli. The recombinant protein is then purified and characterized using various biochemical and biophysical techniques.
Table 2: Production and Purification of Recombinant Proteins
| Step | Description |
|---|---|
| 1 | Gene cloning into an expression vector |
| 2 | Transformation into E. coli |
| 3 | Induction of protein expression |
| 4 | Cell lysis and protein extraction |
| 5 | Purification using affinity chromatography (e.g., His-tag) |
Potential Applications
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Biotechnology: Understanding and manipulating ATP synthase can lead to improvements in photosynthetic efficiency, potentially enhancing crop yields.
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Basic Research: Studying recombinant ATP synthase subunits helps elucidate the mechanisms of photosynthesis and energy conversion in plants.
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Pharmaceuticals: While not directly related to pharmaceuticals, insights into plant biochemistry can inform the development of novel drugs or therapeutic agents.
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized fulfillment.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is requested in advance. Additional fees apply for dry ice shipping.
The specific tag type is determined during the production process. If you require a specific tag, please inform us, and we will prioritize its development.
