Recombinant Oncorhynchus mykiss DNA-binding protein Ikaros (ikzf1), partial

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In Stock
Cat. No.
BT39667
Source
Yeast
Synonyms
ikzf1; ikarosDNA-binding protein Ikaros; Ikaros family zinc finger protein 1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT39667
Source
E.coli
Synonyms
ikzf1; ikarosDNA-binding protein Ikaros; Ikaros family zinc finger protein 1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT39667
Source
E.coli
Synonyms
ikzf1; ikarosDNA-binding protein Ikaros; Ikaros family zinc finger protein 1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT39667
Source
Baculovirus
Synonyms
ikzf1; ikarosDNA-binding protein Ikaros; Ikaros family zinc finger protein 1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT39667
Source
Mammalian cell
Synonyms
ikzf1; ikarosDNA-binding protein Ikaros; Ikaros family zinc finger protein 1
Purity
>85% (SDS-PAGE)
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Description

Overview of IKZF1 (Ikaros)

IKZF1 encodes the DNA-binding transcription factor IKAROS, a critical regulator of lymphoid development. Key features include:

  • Structure: Six zinc finger domains (four N-terminal for DNA binding, two C-terminal for dimerization) .

  • Function: Guides hematopoietic stem cell differentiation into B-cells and regulates transcriptional programs during lymphocyte maturation .

  • Pathology: Somatic IKZF1 deletions/mutations are linked to B-cell acute lymphoblastic leukemia (B-ALL) and immunodeficiency disorders .

Recombinant IKZF1 in Research

While no data exists for Oncorhynchus mykiss (rainbow trout) IKZF1, human recombinant IKZF1 is commercially available. Key specifications from existing sources :

ParameterDetails
SpeciesHuman
Expression HostHEK293T cells
TagC-Myc/DDK
Molecular Weight~57.3 kDa
Purity>80% (SDS-PAGE)
ApplicationTranscription studies, protein interaction assays, antibody production
UniProt IDQ13422

Functional Insights from Human Studies

  • Isoforms: At least 16 splice variants exist, with truncated isoforms (e.g., IK6) acting as dominant-negative regulators .

  • Mechanism: Binds DNA at GGGAA motifs and recruits chromatin-remodeling complexes (e.g., SIN3, NuRD) to repress oncogenes like BCL2L1 .

  • Therapeutic Targeting:

    • Casein kinase 2 (CK2) inhibitors restore IKAROS-mediated transcriptional repression in IKZF1-deleted leukemias .

    • Germline mutations impair drug sensitivity and immune cell development .

Research Gaps and Recommendations

  • Rainbow Trout IKZF1: No studies have characterized this homolog. Comparative genomics suggests conserved zinc finger domains across vertebrates, but functional studies are lacking.

  • Suggested Approaches:

    1. Sequence Alignment: Compare O. mykiss IKZF1 with human/murine sequences to identify conserved domains.

    2. Expression Analysis: Use RNA-seq or CRISPR to study IKZF1 in trout lymphoid tissues.

    3. Recombinant Production: Clone partial IKZF1 sequences into bacterial/insect systems for biochemical assays.

Key Citations

  • Human IKZF1 structure/function .

  • Germline mutations in immunodeficiency .

  • Recombinant protein production .

Product Specs

Form
Lyophilized powder. We will preferentially ship the format we have in stock. If you have special format requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery times vary depending on the purchase method and location. Please consult your local distributor for specific delivery information. All proteins are shipped with standard blue ice packs by default. For dry ice shipping, please contact us in advance, and additional fees will apply.
Notes
Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50% for your reference.
Shelf Life
Shelf life depends on several factors, including storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receiving. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you require a specific tag, please inform us, and we will prioritize developing it.
Synonyms
ikzf1; ikarosDNA-binding protein Ikaros; Ikaros family zinc finger protein 1
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Protein Length
Partial
Purity
>85% (SDS-PAGE)
Species
Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Target Names
ikzf1
Uniprot No.
O13089

Target Background

Function
This protein binds and activates the enhancer (delta-A element) of the CD3-delta gene. It plays a role in T-lymphocyte specification and maturation. It also interacts with a crucial control element in the TDT (terminal deoxynucleotidyltransferase) promoter and with promoters of other genes expressed during early B- and T-cell development. Its function is isoform-specific and is modulated by dominant-negative inactive isoforms.
Database Links

UniGene: Omy.11721

Protein Families
Ikaros C2H2-type zinc-finger protein family
Subcellular Location
Nucleus.
Tissue Specificity
Expression mainly limited to thymus, spleen and pronephros. Very low expression in liver. No expression in testis, brain, eye and muscle.

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