Recombinant Orientia tsutsugamushi Peptide chain release factor 1 (prfA)

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Cat. No.
BT55354
Source
Yeast
Synonyms
prfA; OTT_0012; Peptide chain release factor 1; RF-1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT55354
Source
E.coli
Synonyms
prfA; OTT_0012; Peptide chain release factor 1; RF-1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT55354
Source
E.coli
Synonyms
prfA; OTT_0012; Peptide chain release factor 1; RF-1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT55354
Source
Baculovirus
Synonyms
prfA; OTT_0012; Peptide chain release factor 1; RF-1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT55354
Source
Mammalian cell
Synonyms
prfA; OTT_0012; Peptide chain release factor 1; RF-1
Purity
>85% (SDS-PAGE)
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Description

Overview of Orientia tsutsugamushi Recombinant Proteins

The search results focus on well-characterized O. tsutsugamushi antigens and virulence factors, including:

  • 56-kDa Type-Specific Antigen (TSA56):

    • A major outer membrane protein used for serodiagnosis and vaccine development. It exhibits strain-specific variations but retains conserved domains across genotypes (e.g., Karp, Gilliam, Boryong) .

    • Recombinant TSA56 (rTSA56) demonstrates variable cross-reactivity with antibodies from different strains, limiting broad diagnostic utility .

  • Autotransporter Proteins (ScaA–ScaF):

    • ScaB, a bacterial autotransporter, facilitates host cell adhesion and invasion by interacting with integrin-mediated signaling pathways .

    • ScaA and ScaE are under positive selection, suggesting evolutionary pressure for functional adaptation .

  • Ank5 Effector Protein:

    • A virulence factor that degrades the MHC class I transactivator NLRC5 via ubiquitination, aiding immune evasion .

Potential Misinterpretations of "prfA"

The term "prfA" may refer to:

  • Peptide Chain Release Factors (RF1/RF2):

    • In bacteria, RF1 terminates translation by recognizing stop codons (UAA/UAG). No homologs of RF1 have been reported in O. tsutsugamushi studies.

  • Hypothetical Proteins:

    • O. tsutsugamushi genomes encode numerous uncharacterized proteins. Without genomic or proteomic data specifying "prfA," its existence remains unconfirmed.

Key Research Gaps

  • No studies in the provided sources describe cloning, expression, or functional characterization of a prfA homolog in O. tsutsugamushi.

  • Recombinant protein research on this pathogen focuses on antigens (TSA56, Sca proteins) and virulence effectors (Ank5), not translation termination factors .

Recommendations for Further Investigation

To resolve ambiguities:

  1. Validate the nomenclature "prfA" against O. tsutsugamushi genome databases (e.g., NCBI, UniProt).

  2. Explore proteomic studies for unannotated proteins with RF1-like domains.

  3. Consider potential cross-reactivity studies using polyclonal antibodies against conserved bacterial RF1 sequences.

Table 1: Characterized O. tsutsugamushi Recombinant Proteins

ProteinFunctionDiagnostic/Vaccine RelevanceReferences
TSA56Outer membrane antigenHigh (strain-specific)
ScaBHost cell adhesion/invasionModerate
Ank5Immune evasion (NLRC5 degradation)Experimental

Product Specs

Form
Lyophilized powder. We will preferentially ship the available format. If you have specific format requirements, please note them when ordering.
Lead Time
Delivery times vary by purchase method and location. Consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. For dry ice shipping, contact us in advance; extra fees apply.
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
prfA; OTT_0012; Peptide chain release factor 1; RF-1
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-359
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Orientia tsutsugamushi (strain Ikeda) (Rickettsia tsutsugamushi)
Target Names
prfA
Target Protein Sequence
MSFTVHLNKI LQKYQDLEAD LNGGKLDNKE LALISKEYSN LKPIIEKLNR YLKAHENIKY LQQVIDTEQD LELKSIAEVE LYDTLNYLPK LEEEVKISLL PKESDDHKNA IIEVRAGTGG DEAALFAASL FQMYNRYAER KKWKFELLSI SDTEIGGYKE ASALISGNGV FANLKFESGV HRVQRIPKTE SNGRIHTSAA TVVVLPEAEE VDVKIDAKDL KIDTYRASGA GGQHVNTTDS AVRITHIPTG VVVSQQDEKS QHKNKAKAMK ILYARLYDLE KQKSQQEQAM SRKVQVGTGD RSERIRTYNY PQGRVTDHRI NLTLYKIEEI IQEGKLDEII NNLISENEAK KIADSNIQF
Uniprot No.
B3CQD1

Target Background

Function
Peptide chain release factor 1 terminates translation in response to the stop codons UAG and UAA.
Database Links

KEGG: ott:OTT_0012

Protein Families
Prokaryotic/mitochondrial release factor family
Subcellular Location
Cytoplasm.

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