Recombinant Oryza sativa subsp. japonica CASP-like protein Os02g0134500 (Os02g0134500, LOC_Os02g04180)
Description
Expression Patterns
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Os02g0134500 transcripts are downregulated under certain stress conditions but upregulated in others (e.g., fold changes: -4.45 to +7.15 in RNA-seq data), indicating context-dependent regulation .
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Phylogenetic analysis groups Os02g0134500 within the CASP_like-I subfamily, which is closely related to canonical CASPs involved in barrier formation .
Experimental Use Cases
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Barrier Function Studies: Useful for investigating Casparian strip formation via lignin deposition assays .
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Stress Adaptation: Potential marker for salt or drought tolerance mechanisms in rice .
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Protein-Protein Interaction: His tag facilitates pull-down assays to identify binding partners (e.g., peroxidases or transporters) .
Technical Considerations
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Stability: Hydrophilic nature (grand average hydropathicity >0) and low instability index (<40) suggest robust biochemical handling .
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Limitations: No direct functional studies on this recombinant form exist; inferences rely on homology to characterized CASPs .
Comparative Analysis of CASP Families
| Feature | Rice (OsCASP) | Arabidopsis (AtCASP) |
|---|---|---|
| Gene Count | 41 members | 39 members |
| Key Subfamilies | CASP_like-I/II/III | CASP_like-I/II/III |
| Chromosomal Distribution | Highest on Chr1/12 | Highest on Chr2/5 |
| Root Expression | OsCASP_like11/19 | AtCASP_like1/31 |
Os02g0134500 clusters within the conserved CASP_like-I subfamily, which shows root-specific expression and involvement in ion absorption . Its synteny with Arabidopsis genes suggests evolutionary conservation of function.
Future Directions
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Functional Validation: CRISPR/Cas9 knockout studies to assess roles in root barrier formation.
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Omics Integration: Co-expression analysis with lignin biosynthesis genes (e.g., peroxidases) .
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Biotechnological Applications: Engineering rice varieties with enhanced nutrient use efficiency via CASP modulation .
Product Specs
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