Recombinant Oryza sativa subsp. japonica Cytochrome b6-f complex subunit 4 (petD)
Description
Introduction to Recombinant Oryza sativa subsp. japonica Cytochrome b6-f Complex Subunit 4 (petD)
Recombinant Oryza sativa subsp. japonica Cytochrome b6-f complex subunit 4 (petD) is a protein derived from the cytochrome b6-f complex, which plays a crucial role in photosynthesis by facilitating electron transfer between photosystem II (PSII) and photosystem I (PSI) in chloroplasts. This protein is essential for cyclic electron flow around PSI and state transitions, processes that optimize photosynthetic efficiency under varying light conditions.
Function and Importance
The cytochrome b6-f complex, of which subunit 4 (petD) is a part, is integral to the photosynthetic electron transport chain. It mediates the transfer of electrons from plastoquinol to plastocyanin, generating a proton gradient across the thylakoid membrane that drives ATP synthesis. Subunit IV (petD) is known to be involved in proton translocation and electron transfer activities within the complex .
Structure and Characteristics
Subunit IV (petD) of the cytochrome b6-f complex is a single-pass membrane protein with a molecular mass of approximately 17 kDa . It is located in the thylakoid membrane of chloroplasts and is part of the larger cytochrome b6-f complex, which functions as a dimer . The recombinant version of this protein is often expressed in Escherichia coli and may be tagged with a His-tag for purification purposes .
Recombinant Production and Applications
Recombinant Oryza sativa subsp. japonica Cytochrome b6-f complex subunit 4 (petD) is produced using recombinant DNA technology, where the gene encoding the protein is inserted into a suitable expression vector and expressed in a host organism like E. coli. This recombinant protein is used in research studies to understand the structure-function relationships of the cytochrome b6-f complex and its role in photosynthesis. It is also available commercially for research purposes, with prices varying depending on the supplier .
Research Findings
Recent studies have highlighted the importance of interactions between subunits within the cytochrome b6-f complex. For example, salt bridge formation between cytochrome b6 and subunit IV is crucial for the assembly and stability of the complex . Additionally, subunit IV is sensitive to trypsin digestion, which affects the electron transfer activity of the complex .
Table 1: Characteristics of Recombinant Oryza sativa subsp. japonica Cytochrome b6-f Complex Subunit 4 (petD)
| Characteristic | Description |
|---|---|
| Molecular Mass | Approximately 17 kDa |
| Expression Host | Escherichia coli |
| Tag | His-tag for purification |
| Function | Electron transfer and proton translocation in photosynthesis |
| Location | Thylakoid membrane of chloroplasts |
Table 2: Suppliers and Pricing
| Supplier | Price |
|---|---|
| MyBioSource.com | $1,435.00 |
| Creative Biomart | Not specified |
Table 3: Key Interactions and Functions
| Subunit | Interaction/Function |
|---|---|
| Cytochrome b6 (PetB) | Salt bridge formation with PetD essential for complex assembly |
| Subunit IV (PetD) | Proton translocation and electron transfer |
References MyBioSource.com. Recombinant Oryza sativa subsp. japonica Cytochrome b6-f complex subunit 4 (petD). PubMed. The catalytic role of subunit IV of the cytochrome b6-f complex. PubMed. The stromal side of the cytochrome b6f complex regulates state transitions. Creative Biomart. Recombinant Full Length Oryza Sativa Subsp. Japonica Cytochrome B6-F Complex Subunit 4(Petd) Protein, His-Tagged. UniProt/SWISS-PROT: CYF_ORYSJ. PMC. Structure of the Cytochrome b6f Complex: Quinone Analogue Binding Sites. CBM15. ELISA Recombinant Oryza sativa subsp. japonica Cytochrome b6-f complex subunit 4(petD). STRING interaction network. petC protein (Oryza sativa Japonica).
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Tag type is determined during production. If you require a specific tag, please inform us; we will prioritize its development.
Target Background
Component of the cytochrome b6-f complex. This complex mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.
KEGG: osa:3131404
STRING: 39947.LOC_Osp1g00650.1
