Recombinant Oryza sativa subsp. japonica Lipoyl synthase 2, chloroplastic (LIP1P-2)

Introduction to Recombinant Oryza sativa subsp. japonica Lipoyl Synthase 2, Chloroplastic (LIP1P-2)

Recombinant Oryza sativa subsp. japonica Lipoyl Synthase 2, chloroplastic (LIP1P-2) is a recombinant protein derived from the Japanese rice subspecies. This enzyme plays a crucial role in the biosynthesis of lipoic acid, a vital cofactor for several key metabolic enzymes. LIP1P-2 is specifically localized to the chloroplasts, where it catalyzes the radical-mediated insertion of two sulfur atoms into the octanoyl moiety of lipoate-dependent enzymes, converting them into lipoylated derivatives .

Biochemical Characteristics

• Function: LIP1P-2 is involved in the synthesis of lipoic acid, which is essential for the proper functioning of enzymes like pyruvate dehydrogenase and alpha-ketoglutarate dehydrogenase.

• Localization: Chloroplastic, indicating its role in plant metabolism within the chloroplasts.

• Expression System: Produced in E. coli, which is a common host for recombinant protein production due to its well-understood genetics and high yield .

Sequence and Structure

The sequence of LIP1P-2 includes a specific arrangement of amino acids that facilitate its enzymatic activity. The full-length mature protein sequence is crucial for its function in converting octanoylated domains into lipoylated derivatives .

Interaction Network

LIP1P-2 interacts with other proteins involved in metabolic pathways. Predicted functional partners include lipoate-protein ligase B and other lipoyl synthases, suggesting a complex network of interactions within the chloroplast .

References

1. Cusabio. Recombinant Oryza sativa subsp. japonica Lipoyl synthase 2, chloroplastic (LIP1P-2) [CSB-EP740350OFG]. [Accessed 2025].

2. PMC. Lip1p: a novel subunit of acyl-CoA ceramide synthase. [Accessed 2025].

3. MDPI. Rhizopus oryzae Lipase, a Promising Industrial Enzyme: Biochemical Characteristics, Production and Biocatalytic Applications. [Accessed 2025].

4. STRING. LIP1P-2 protein (Oryza sativa Japonica) - STRING interaction network. [Accessed 2025].

5. PMC. Yeast Cells Lacking All Known Ceramide Synthases Continue to Make Ceramides. [Accessed 2025].

6. PMC. Identification, Biological Function Profiling and Biosynthesis of Secondary Metabolites in Medicinal Orchids. [Accessed 2025].

7. GenScript. LOC4325595 gene cDNA ORF clone, Oryza sativa Japonica Group. [Accessed 2025].

8. eLife. TORC2-dependent protein kinase Ypk1 phosphorylates ceramide synthase components to regulate sphingolipid homeostasis. [Accessed 2025].

9. Cusabio. Recombinant Oryza sativa subsp. japonica Soluble starch synthase 2-2, chloroplastic/amyloplastic (SSII-2), partial. [Accessed 2025].