Recombinant Oryzias latipes Hepatocyte nuclear factor 3-beta (foxa2)

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Cat. No.
BT49232
Source
Yeast
Synonyms
foxa2; hnf3bHepatocyte nuclear factor 3-beta; HNF-3-beta; HNF-3B; Forkhead box protein A2; Me-HNF3B
Purity
>85% (SDS-PAGE)
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Cat. No.
BT49232
Source
E.coli
Synonyms
foxa2; hnf3bHepatocyte nuclear factor 3-beta; HNF-3-beta; HNF-3B; Forkhead box protein A2; Me-HNF3B
Purity
>85% (SDS-PAGE)
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Cat. No.
BT49232
Source
E.coli
Synonyms
foxa2; hnf3bHepatocyte nuclear factor 3-beta; HNF-3-beta; HNF-3B; Forkhead box protein A2; Me-HNF3B
Purity
>85% (SDS-PAGE)
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Cat. No.
BT49232
Source
Baculovirus
Synonyms
foxa2; hnf3bHepatocyte nuclear factor 3-beta; HNF-3-beta; HNF-3B; Forkhead box protein A2; Me-HNF3B
Purity
>85% (SDS-PAGE)
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Cat. No.
BT49232
Source
Mammalian cell
Synonyms
foxa2; hnf3bHepatocyte nuclear factor 3-beta; HNF-3-beta; HNF-3B; Forkhead box protein A2; Me-HNF3B
Purity
>85% (SDS-PAGE)
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Description

Overview of FoxA2 and Its Biological Role

FoxA2, a member of the forkhead box (Fox) transcription factor family, functions as a "pioneer factor" that binds compacted chromatin to enable transcriptional activation of target genes . Key roles include:

  • Embryonic development: Essential for endoderm-derived organ formation (e.g., liver, pancreas) .

  • Metabolic regulation: Modulates glucose homeostasis, lipid metabolism, and bile acid synthesis .

  • Chromatin remodeling: Displaces nucleosomes to allow access for other transcription factors .

Recombinant Oryzias latipes FoxA2: Production and Characteristics

The recombinant variant is synthesized using yeast (Saccharomyces cerevisiae) as the host organism . Key specifications include:

ParameterDetail
OriginOryzias latipes (Japanese medaka)
Expression HostYeast
Purity>90% (verified by SDS-PAGE)
TagC-terminal His tag
ApplicationsELISA, functional studies (expected but not yet validated)
Storage-80°C; stable for 12 months (avoid freeze-thaw cycles)

Production Workflow:

  1. Gene cloning of FoxA2 from Oryzias latipes.

  2. Transformation into yeast for expression.

  3. Affinity purification using nickel-charged resins for His-tagged protein .

Functional Insights from Cross-Species Studies

SpeciesKey FindingImplication for O. latipes Studies
MouseFoxA2 deficiency causes age-related obesity and hepatic steatosis .Highlights metabolic regulatory pathways to investigate.
HumanFoxA2 regulates lipoprotein lipase (LPL), influencing triglyceride uptake .Suggests roles in lipid metabolism for medaka FoxA2.
RatFoxA2 interacts with nuclear receptors (FXR, LXR) to modulate bile acid genes .Supports exploration of detoxification pathways in fish.

Anticipated Research Applications

  • Developmental biology: Study conserved mechanisms in endoderm patterning.

  • Toxicology: Assess pollutant impacts on hepatic gene regulation.

  • Evolutionary studies: Compare chromatin remodeling across vertebrates.

Challenges and Future Directions

  • Functional validation: Current data rely on extrapolation from mammalian models; direct assays (e.g., chromatin immunoprecipitation) are needed .

  • Structural analysis: The winged helix DNA-binding domain is conserved, but medaka-specific variations may exist .

  • Disease modeling: Potential to study FoxA2’s role in fish hepatic disorders mirroring human NAFLD/NASH .

Product Specs

Form
Lyophilized powder. We will ship the available format, but please note any format requirements when ordering, and we will try to accommodate them.
Lead Time
Delivery times vary by purchase method and location. Consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. Please contact us in advance for dry ice shipping, which incurs extra fees.
Notes
Avoid repeated freezing and thawing. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute the protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, temperature, and protein stability. Liquid form typically lasts 6 months at -20°C/-80°C. Lyophilized form typically lasts 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon arrival. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
foxa2; hnf3bHepatocyte nuclear factor 3-beta; HNF-3-beta; HNF-3B; Forkhead box protein A2; Me-HNF3B
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-415
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Oryzias latipes (Japanese rice fish) (Japanese killifish)
Target Names
foxa2
Target Protein Sequence
MMLGAVKMEG HEHTDWSTYY GEPECYTSVG NMNTGLGMNS MNTYMSMSGM STTANMTANS MNMSYVNTGM SPSMTGMSPG TGAMNGMGAG MTAMSTALSP SMSPMTGQPG SMNALTSYTN MNAMSPIYGQ SNINRSRDPK TYRRSYTHAK PPYSYISLIT MAIQQSPSKM LTLAEIYQWI MDLFPFYRQN QQRWQNSIRH SLSFNDCFLK VPRSPDKPGK GSFWTLHPDS GNMFENGCYL RRQKRFKCEK KMSMKEPGRK GGDGGSANSS SDSCNGNESP HSNSSSGEHK RSLSDMKGSQ ALSPEHTAPS PVSQGQHLMS QHHSVLAHEA HLKPEHHYSF NHPFSINNLM SSEQQHHKMD LKTYEQVMHY SGYGSPMTGA LSMGSMAGKA GLDSASIPDT SYYQGVYSRP IMNSS
Uniprot No.
O42097

Target Background

Function
Transcription activator for several liver genes. Interacts with their cis-acting regulatory regions.
Database Links

KEGG: ola:100049256

STRING: 8090.ENSORLP00000021768

UniGene: Ola.93

Subcellular Location
Nucleus.

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