Recombinant Oscillatoria agardhii Gas vesicle protein C (gvpC)

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Description

Functional Role in Gas Vesicle Assembly

Recombinant GvpC interacts with multiple proteins to orchestrate gas vesicle biogenesis:

  • GvpA Binding: Stabilizes the ribbed structure of GvpA multimers via N-terminal repeats .

  • Accessory Protein Interactions:

    • GvpN (AAA-ATPase): Binds C-terminal GvpC to regulate ATP-dependent vesicle growth .

    • GvpO: Enhances GvpA multimerization and interacts with GvpC’s C-terminus .

Table 2: Protein Interaction Network of GvpC

Interacting ProteinBinding Region on GvpCFunctional Role
GvpAN-terminal repeatsStructural reinforcement
GvpNC-terminal domainATP hydrolysis for vesicle expansion
GvpOC-terminal domainChaperone-like stabilization

Genetic and Biotechnological Applications

  • Genetic Clusters: The gvpACNO operon encodes GvpC, alongside GvpA, GvpN, and GvpO . Recombinant systems often use modified clusters for heterologous expression .

  • Biomedical Potential: Engineered gas vesicles incorporating recombinant GvpC are explored as ultrasound contrast agents .

Research Gaps and Future Directions

  • Mechanistic Details: ATPase activity of GvpN and its regulation of GvpC binding remain poorly understood .

  • Taxonomic Clarification: Oscillatoria agardhii is often conflated with Planktothrix spp.; genomic comparisons are needed .

Product Specs

Form
Lyophilized powder. We will ship the format we have in stock. If you have special format requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery time varies depending on purchasing method and location. Consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. For dry ice shipping, please contact us in advance; additional charges apply.
Notes
Avoid repeated freezing and thawing. Working aliquots are stable at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50% for your reference.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form is stable for 6 months at -20°C/-80°C, and the lyophilized form is stable for 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you require a specific tag, please inform us, and we will prioritize developing it.
Synonyms
gvpC; Gas vesicle protein C; Fragment
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-28
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Planktothrix agardhii (Oscillatoria agardhii)
Target Names
gvpC
Target Protein Sequence
ALKDKNQLDR IGIVQGVILX QQIVITAL
Uniprot No.

Target Background

Function
May stabilize gas vesicle membranes. Gas vesicles are small, hollow, gas-filled protein structures found in various planktonic microorganisms. They enable the organism to position itself at the optimal depth for growth.
Protein Families
Gas vesicle protein type C family
Subcellular Location
Vacuole, gas vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Binds to the external surface of the gas vesicle membrane.

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