Recombinant Panax ginseng NAD (P)H-quinone oxidoreductase subunit 6, chloroplastic (ndhG)
Description
Functional Role in Chloroplast NDH Complex
The NDH complex operates in cyclic electron flow around photosystem I (PSI) and chlororespiration . ndhG contributes to:
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Electron Shuttling: Transfers electrons via FMN and Fe-S centers to plastoquinone .
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Proton Gradient Formation: Couples redox reactions to proton translocation, supporting ATP synthesis .
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Stress Adaptation: Enhances plant resilience under environmental stress by balancing ATP/NADPH ratios .
Subunit Interactions:
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ndhG associates with hydrophobic subunits (ndhA–ndhF) in the membrane subcomplex .
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Stability of ndhG depends on proper folding of ndhH, mediated by chaperonins like Cpn60β4 .
Amino Acid Sequence Alignment
Both sequences feature transmembrane helices and conserved residues critical for quinone binding .
Purification and Activity Assays
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Purity: Recombinant ndhG proteins exhibit >90% purity via SDS-PAGE .
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Stability: Lyophilized ndhG retains activity at -80°C but degrades with repeated freeze-thaw cycles .
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Electron Transport Kinetics:
Mutational Studies
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Arabidopsis ndhG Knockouts: Impaired NDH activity, reduced PSI cyclic electron flow, and increased sensitivity to high light .
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Cyanobacterial Homologs: ndhG deletion disrupts NDH-1L complex assembly, abolishing respiratory electron transport .
Comparative Analysis with Other Subunits
The chloroplast NDH complex comprises 11 plastid-encoded subunits (ndhA–ndhK) and nuclear-encoded auxiliary proteins . Key distinctions:
Implications for Panax ginseng Research
While recombinant Panax ginseng ndhG remains understudied, its homologs suggest potential roles in:
Product Specs
Note: We will prioritize shipping the format that we have in stock. However, if you have a specific requirement for the format, please indicate your preference when placing your order. We will prepare the product according to your request.
Note: All of our proteins are shipped with standard blue ice packs by default. If you require dry ice shipping, please inform us in advance as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
