Recombinant Pasteurella multocida Glycerol-3-phosphate acyltransferase (plsY)
Description
Enzymatic Mechanism and Catalytic Motifs
PlsY belongs to the acyl-phosphate:G3P acyltransferase family. Structural studies on homologs (e.g., Streptococcus pneumoniae) reveal:
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Membrane topology: Five transmembrane segments with conserved cytoplasmic motifs .
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Critical residues:
Role in Bacterial Physiology
PlsY is pivotal for:
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Phospholipid biosynthesis: Initiates phosphatidic acid formation, a precursor for membrane lipids .
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Biofilm regulation: Mutants with disrupted lipid biosynthesis in P. multocida show altered biofilm formation, linking PlsY to bacterial persistence in hosts .
Vaccine Development
While PlsY itself is not directly tested as a vaccine antigen, studies on P. multocida recombinant proteins (e.g., PlpE, OmpH) highlight the potential of membrane-associated enzymes as vaccine candidates. For example:
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Subunit vaccines combining outer membrane proteins (OmpH) and lipoproteins (PlpE) confer 83–100% protection in ducks .
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Recombinant PlsY could serve as a target for adjuvant-based vaccines due to its surface accessibility and conserved motifs .
Antibiotic Resistance Studies
PlsY’s role in membrane integrity makes it a potential target for disrupting bacterial lipid biosynthesis. Inhibitors targeting its catalytic motifs could reduce virulence .
Future Directions
Product Specs
Note: While we will prioritize shipping the format currently in stock, we are happy to accommodate specific format requests. Please indicate your preferred format in the order notes, and we will fulfill your requirements.
Note: All protein shipments are standardly packaged with blue ice packs. If you require dry ice shipping, please communicate this need in advance. Additional fees may apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: pmu:PM1696
STRING: 272843.PM1696
