Recombinant Perisphaeria cf. substylifera Periviscerokinin-2

Shipped with Ice Packs
In Stock

Description

Physiological Studies

  • Tick Feeding and Reproduction: CAPA neuropeptides, including PVK-2, are implicated in regulating feeding, survival, and reproduction in ticks. Silencing the CAPA receptor in Rhipicephalus microplus reduced female survival, egg mass weight, and hatching rates .

  • Fluid Homeostasis: PVK-2 homologs in insects and ticks influence Malpighian tubule function, suggesting a role in osmotic balance .

Receptor Studies

  • The CAPA/PVK receptor belongs to the PRX-amide GPCR family. Recombinant PVK-2 is used to study receptor-ligand interactions, particularly in tick synganglion and salivary glands .

Comparative Analysis of CAPA Neuropeptides

SpeciesNeuropeptideFunctionReceptor Activation
Perisphaeria cf. substyliferaPVK-2Myotropic activity, diuresisPRX-amide GPCRs
Rhipicephalus microplusRhimi-CAPA-PVK1/2Feeding regulation, egg developmentRhimi-CAP2bR
Periplaneta americanaPVK-2Hindgut muscle contractionNot fully characterized

Usage Notes

  • Stability: Avoid repeated freeze-thaw cycles; working aliquots stored at 4°C retain activity for one week .

  • Experimental Design: Used in functional assays (e.g., calcium flux assays) to study CAPA receptor signaling in arthropods .

Product Specs

Form
Lyophilized powder. We will ship the format in stock. If you have special format requirements, please note them when ordering.
Lead Time
Delivery time varies by purchase method and location. Consult local distributors for specifics. Proteins are shipped with blue ice packs. Request dry ice in advance (extra fees apply).
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you have a specific tag type requirement, please inform us and we will prioritize its development.
Synonyms
Periviscerokinin-2; PerSu-PVK-2
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-11
Protein Length
Cytoplasmic domain
Purity
>85% (SDS-PAGE)
Species
Perisphaeria cf. substylifera (strain SR-2005) (Cockroach)
Target Protein Sequence
GSSGLISMPR V
Uniprot No.

Target Background

Function
Mediates visceral muscle contractile activity (myotropic activity).
Protein Families
Periviscerokinin family
Subcellular Location
Secreted.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
© Copyright 2025 TheBiotek. All Rights Reserved.