Recombinant Petaurus breviceps NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L)
Description
Biological Role
MT-ND4L is a core subunit of complex I (NADH:ubiquinone oxidoreductase), which catalyzes the transfer of electrons from NADH to ubiquinone in the mitochondrial inner membrane . This process generates a proton gradient essential for ATP synthesis during oxidative phosphorylation. Mutations in homologous human MT-ND4L genes are linked to Leber hereditary optic neuropathy, a mitochondrial disorder causing vision loss .
Expression and Purification
The recombinant protein is produced in E. coli using optimized conditions to ensure proper folding and solubility. Key steps include:
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Cloning: Insertion of the MT-ND4L gene into a plasmid vector.
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Fermentation: High-density E. coli cultures for protein expression.
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Purification: Affinity chromatography (via His-tag) followed by size-exclusion chromatography .
Cross-Species Comparisons
Functional Studies
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Complex I Assembly: ND4L is essential for the assembly of the 950-kDa complex I holocomplex. Its absence disrupts electron transport and ATP production .
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ELISA Development: Recombinant MT-ND4L serves as an antigen in ELISA kits for detecting anti-complex I antibodies or studying mitochondrial dysfunction .
Therapeutic Relevance
While the recombinant protein itself is not therapeutic, it aids in understanding mitochondrial complex I deficiencies. For example:
Product Specs
Note: We will prioritize shipping the format we currently have in stock. However, if you have a specific format requirement, please indicate it when placing your order. We will fulfill your request if possible.
Note: All our proteins are shipped with standard blue ice packs by default. If you require dry ice shipping, please notify us in advance, as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
