Recombinant Phaeodactylum tricornutum Cytochrome b559 subunit alpha (psbE)
Description
Production and Purification
Recombinant psbE is produced via bacterial expression systems, typically in E. coli, with optimized protocols for yield and stability:
| Parameter | Specification |
|---|---|
| Purity | >90% (SDS-PAGE verified) |
| Storage Buffer | Tris/PBS-based buffer, 6% trehalose, pH 8.0 |
| Stability | Lyophilized powder; store at -20°C/-80°C (avoid repeated freeze-thaw cycles) |
| Reconstitution | Deionized water (0.1–1.0 mg/mL), with 5–50% glycerol for long-term storage |
Protein reconstitution guidelines emphasize careful handling to prevent aggregation .
Role in PSII Assembly and Photoprotection
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Structural Importance: The alpha subunit’s Arg residues (e.g., Arg7, Arg8, Arg18) interact with heme propionates, influencing redox properties and PSII stability .
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Functional Redundancy: Mutants lacking heme coordination retained PSII activity, suggesting compensatory mechanisms in T. elongatus .
Expression in Diatoms
While recombinant psbE is produced in E. coli, Phaeodactylum tricornutum itself is a model for algal biotechnology:
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Protein Expression: This diatom has been engineered to produce complex proteins (e.g., IgG antibodies, HBsAg) with proper post-translational modifications .
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Potential for psbE: Though not directly reported, its genetic toolkit could enable native psbE overexpression for studying photosynthetic pathways .
Comparative Biochemical Studies
Research on Cytochrome b559 in other organisms (e.g., Synechocystis, T. elongatus) highlights its dual roles:
Biotechnological Relevance
The recombinant psbE protein serves as a tool for:
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Structural Biology: Studying PSII’s reaction center dynamics and interactions with light-harvesting complexes (e.g., FCPs) .
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Redox Biochemistry: Investigating Cytochrome b559’s role in superoxide dismutase activity and quinone binding .
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Algal Engineering: Leveraging P. tricornutum’s capacity for heterologous protein production to explore synthetic biology applications .
Challenges and Future Directions
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have a specific format requirement, please indicate it in your order notes. We will accommodate your request to the best of our ability.
Note: All our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance. Additional fees may apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
