Recombinant Phalaenopsis aphrodite subsp. formosana Photosystem II CP47 chlorophyll apoprotein (psbB)

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Cat. No.

BT2147671

Source

in vitro E.coli expression system

Synonyms

psbB; Photosystem II CP47 reaction center protein; PSII 47 kDa protein; Protein CP-47

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Description

Functional Role in Photosynthesis

CP47 is a core component of PSII, binding chlorophyll a and facilitating electron transfer. Key findings:

Structural Role: Forms a heterodimer with CP43, stabilizing the oxygen-evolving complex (OEC) and reaction center .
Mutant Studies:
- In Arabidopsis, mutations in auxiliary proteins (e.g., FPB1 or PAM68) impair CP47 synthesis, leading to PSII assembly defects .
- Variegated P. aphrodite subsp. formosana mutants exhibit reduced PsbP (OEC subunit) expression, disrupting thylakoid folding and chlorophyll accumulation .

Table 1: Genomic Features of Phalaenopsis CP47

Feature	Detail	Source
Gene Length (bp)	1,524 (coding sequence)
Protein Length (aa)	508
Chloroplast Genome Size	148,964 bp (P. aphrodite subsp. formosana)
Conserved Domains	6 transmembrane helices; chlorophyll-binding sites

Research Applications

Photosynthesis Studies: Used to investigate PSII assembly, chlorophyll-protein interactions, and electron transport mechanisms .
Mutagenesis Analysis: Recombinant CP47 enables site-directed mutagenesis to probe residues critical for PSII stability .
Biotechnological Tools: Serves as a reference protein for optimizing chloroplast gene expression in orchids .

Key Research Findings

Post-Transcriptional Regulation: Alternative splicing and polyadenylation of PsbO/PsbP pre-mRNAs in P. aphrodite subsp. formosana variegated mutants correlate with CP47 destabilization .
Viral Interactions: Cymbidium mosaic virus (CymMV) infection exacerbates CP47 deficiency in variegated phenotypes .
Evolutionary Conservation: CP47 (COG5717) is conserved across 70+ species, with a median protein length of 511.79 aa .

Product Specs

Form

Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference during ordering for customized preparation.

Lead Time

Delivery times vary depending on the purchasing method and location. Please consult your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.

Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Centrifuge the vial briefly before opening to consolidate the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50% and can serve as a reference.

Shelf Life

Shelf life depends on various factors including storage conditions, buffer composition, temperature, and the protein's inherent stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.

Storage Condition

Upon receipt, store at -20°C/-80°C. Aliquot to prevent repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing.
The tag type is determined during production. If you require a specific tag, please inform us; we will prioritize its development.

Synonyms

psbB; Photosystem II CP47 reaction center protein; PSII 47 kDa protein; Protein CP-47

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-508

Protein Length

full length protein

Species

Phalaenopsis aphrodite subsp. formosana (Moth orchid)

Target Names

psbB

Target Protein Sequence

MGLPWYRVHTVVLNDPGRLLSVHIMHTALVSGWAGSMALYELAVFDPSDPVLDPMWRQGM FVIPFMTRLGITNSWGGWSISGGTITNPGIWSYEGVAGAHILFSGLCFLAAIWHWVYWDL EIFCDERTGKPSLDLPKIFGIHLFLSGIACFGFGAFHVTGLYGPGIWVSDPYGLTGKVQS VNPAWGAEGFDPFVPGGIASHHIAAGTLGILAGLFHLSVRPPQRLYKGLRMGNIETVLSS SIAAVFFAAFVVAGTMWYGSATTPIELFGPTRYQWDQGYFQQEIYRRVGAGLAENLSLSE AWSKIPEKLAFYDYIGNNPAKGGLFRAGSMDNGDGIAVGWLGHPIFRDKEGHELFVRRMP TFFETFPVVLVDADGIVRADVPFRRAESKYSVEQVGVTVEFYGGELNGVIYSDPATVKKY ARRAQLGEIFELDRATLKSDGVFRSSPRGWFTFGHATFALLFFFGHIWHGARTLFRDVFA GIDPDLDAQVEFGAFQKLGDPTTRRQVV

Uniprot No.

Q3BAL2

Target Background

Function

A core component of the Photosystem II (PSII) complex. It binds chlorophyll and facilitates the primary light-induced photochemical reactions within PSII. PSII functions as a light-driven water:plastoquinone oxidoreductase, utilizing light energy to extract electrons from H₂O, generating O₂ and a proton gradient for subsequent ATP synthesis.

Protein Families

PsbB/PsbC family, PsbB subfamily

Subcellular Location

Plastid, chloroplast thylakoid membrane; Multi-pass membrane protein.

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Recombinant Phalaenopsis aphrodite subsp. formosana Photosystem II CP47 chlorophyll apoprotein (psbB)

Description

Functional Role in Photosynthesis

Table 1: Genomic Features of Phalaenopsis CP47

Research Applications

Key Research Findings

Product Specs

Target Background

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