Recombinant Phoca sibirica Nuclear receptor subfamily 1 group I member 3 (NR1I3)

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Cat. No.
BT41087
Source
Yeast
Synonyms
NR1I3; CAR; Nuclear receptor subfamily 1 group I member 3; Constitutive androstane receptor; CAR
Purity
>85% (SDS-PAGE)
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Cat. No.
BT41087
Source
E.coli
Synonyms
NR1I3; CAR; Nuclear receptor subfamily 1 group I member 3; Constitutive androstane receptor; CAR
Purity
>85% (SDS-PAGE)
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Cat. No.
BT41087
Source
E.coli
Synonyms
NR1I3; CAR; Nuclear receptor subfamily 1 group I member 3; Constitutive androstane receptor; CAR
Purity
>85% (SDS-PAGE)
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Cat. No.
BT41087
Source
Baculovirus
Synonyms
NR1I3; CAR; Nuclear receptor subfamily 1 group I member 3; Constitutive androstane receptor; CAR
Purity
>85% (SDS-PAGE)
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Cat. No.
BT41087
Source
Mammalian cell
Synonyms
NR1I3; CAR; Nuclear receptor subfamily 1 group I member 3; Constitutive androstane receptor; CAR
Purity
>85% (SDS-PAGE)
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Description

Definition and Background

Recombinant Phoca sibirica Nuclear Receptor Subfamily 1 Group I Member 3 (NR1I3) is a genetically engineered protein derived from the Baikal seal (Phoca sibirica). It belongs to the constitutive androstane receptor (CAR) family, a key regulator of xenobiotic and endobiotic metabolism in mammals. CAR proteins are transcription factors that activate detoxification pathways, including cytochrome P450 enzymes and conjugation enzymes, in response to harmful substances like drugs, pollutants, and endogenous metabolites .

The recombinant version (ABIN1611136) is produced via yeast expression systems and tagged with a His-tag for purification and functional studies . Its amino acid sequence (1-348) exhibits high homology with CAR proteins from other mammals, including humans (84% identity) and mice (74%) .

2.1. Protein Structure

  • Molecular Weight: 39.4 kDa (predicted from 348 amino acids) .

  • Domains: Includes a DNA-binding domain (DBD) and ligand-binding domain (LBD), with a conserved activation function-2 (AF-2) transactivation domain .

  • Tag: His-tag for affinity purification .

2.2. Biological Function

CAR proteins regulate detoxification by binding to response elements in target genes, such as:

  • CYP2B/2C/3A (cytochrome P450 enzymes) .

  • Sulfotransferases and glutathione-S-transferases .

  • Bilirubin clearance and xenobiotic metabolism .

2.3. Ligand Interactions

  • Endogenous Ligands: Androstanes (e.g., androstenol, DHEA) .

  • Xenobiotics: Activated by compounds like TCPOBOP (mouse CAR) and CITCO (human CAR) .

Expression and Tissue Distribution

  • Baikal Seal: Expressed in liver microsomes, with mRNA detected in multiple tissues .

  • Recombinant Protein: Produced in yeast systems for research use .

4.1. Toxicology Studies

  • Persistent Organic Pollutants (POPs): CAR expression correlates with CYP enzyme activity in Baikal seals exposed to PCBs and DDTs .

  • Species-Specific Activation: Seal CAR exhibits divergent ligand specificity compared to human and mouse CAR (e.g., TCPOBOP activates mouse but not seal CAR) .

4.2. Functional Assays

  • Reporter Gene Assays: Used to study ligand-dependent transactivation (e.g., TCPOBOP and CITCO) .

  • Protein Interactions: Analyzed via Western blot and co-immunoprecipitation .

4.3. Comparative Evolutionary Analysis

SpeciesCAR Identity (%)Key Ligands
Human84%CITCO, bilirubin
Mouse74%TCPOBOP
Baikal Seal–POPs, androstanes

5.1. Amino Acid Sequence Alignment

DomainBaikal Seal CARHuman CARMouse CAR
DBD (aa 1-66)95% identity84% identity74% identity
LBD (aa 67-348)83% identity80% identity70% identity

5.2. Applications in Research

Use CaseMethodOutcome
Xenobiotic sensingReporter assaysEC50 values for ligand activation
CYP enzyme regulationqRT-PCRCorrelation with POP exposure
Protein purificationHis-tag affinity>90% purity

Product Specs

Form
Lyophilized powder. We will preferentially ship the available format, but if you have specific requirements, please note them when ordering, and we will accommodate your request.
Lead Time
Delivery time varies based on purchasing method and location. Please consult your local distributors for specific delivery times. All proteins are shipped with standard blue ice packs. For dry ice shipping, please contact us in advance as extra fees apply.
Notes
Avoid repeated freezing and thawing. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. Adding 5-50% glycerol (final concentration) is recommended for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on several factors: storage conditions, buffer composition, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon arrival. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
NR1I3; CAR; Nuclear receptor subfamily 1 group I member 3; Constitutive androstane receptor; CAR
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-348
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Pusa sibirica (Baikal seal) (Phoca sibirica)
Target Names
NR1I3
Target Protein Sequence
MASGEDGPRS CMVCGDRATG YHFHALTCEG CKGFFRRTVS KNTGLTCPFA GSCKVNKAQR RHCPACRLQK CLDAGMKKEM ILSAEALAQR RAKQAQRRAQ WAAVQLSKGQ QELVQTLLGA HARHVGTMFD QFVQFRPPAH LFIHHQRLPI PLPPLPLLEH FAEVNTFMVQ QVIKFTKDLP LFRSLPMEDQ ISLLKGAAVE ICHIALNTTF CLQTRNFLCG PLRYTLEDGV HVGFQEEFLE LLFRFHATLR RFQLQEPEYV LMAAMALFSP DRPGVTQKEE IDRLQEMMAL TLQSYIKGQP PRHRDRFLYA KLLGLLAELR SINNAYGYQI QHIQGLSAMM PLLQEICS
Uniprot No.
P62045

Target Background

Function
Binds to and activates retinoic acid response elements, controlling the expression of retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Also transactivates the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element.
Protein Families
Nuclear hormone receptor family, NR1 subfamily
Subcellular Location
Nucleus. Cytoplasm. Cytoplasm, cytoskeleton.

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