Recombinant Photobacterium profundum 50S ribosomal protein L29 (rpmC)
Description
Definition and Functional Overview
Ribosomal protein L29 is a structural component of the 50S ribosomal subunit in bacteria, critical for stabilizing the ribonucleoprotein complex and facilitating translation. In E. coli, it binds 23S rRNA and interacts with the polypeptide exit tunnel, playing a role in nascent protein folding by contacting the trigger factor chaperone . While Photobacterium profundum is a deep-sea bacterium with unique adaptations to high pressure and low temperature, its L29 homolog is not explicitly studied in the provided sources.
Biochemical Characteristics
Genetic and Adaptive Context in Photobacterium profundum
P. profundum is a piezopsychrophile (pressure- and cold-tolerant) with a unique genome structure:
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Genome Composition: Two circular chromosomes (4.1 Mbp and 2.2 Mbp) and a plasmid (80 kbp) .
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Ribosomal Adaptations:
Key Findings from Transposon Mutagenesis:
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Mutations in ribosome-related genes (e.g., rpoX, RNA polymerase sigma factors) impair growth at low temperatures and high pressures .
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Stress-response regulators (e.g., ToxR, DnaK) modulate ribosomal protein expression in response to environmental changes .
Recombinant Production and Applications
While no P. profundum L29 recombinant is documented, the E. coli version is widely used:
Potential P. profundum Applications:
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Biotechnology: High-pressure environments require stable ribosomal proteins. A P. profundum L29 recombinant could be engineered for extremophile-based industrial processes.
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Structural Biology: Comparative studies with E. coli L29 could reveal pressure-adaptive residues in P. profundum.
Research Gaps and Future Directions
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Lack of Direct Data: No studies explicitly address P. profundum L29.
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Hypothesized Role: Based on conserved ribosomal functions, L29 likely stabilizes the 50S subunit and interacts with trigger factor in P. profundum.
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Pressure Adaptation: Mutagenesis studies in P. profundum highlight ribosomal proteins as critical for survival under deep-sea conditions .
Product Specs
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