Recombinant Photobacterium profundum tRNA (Ile)-lysidine synthase (tilS)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: We will prioritize shipment based on available stock. Should you require a specific format, please specify this during order placement.
Note: We will prioritize shipment based on available stock. Should you require a specific format, please specify this during order placement.
Lead Time
Delivery times vary depending on the purchase method and location. Please consult your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs unless otherwise requested. Dry ice shipment requires prior communication and incurs additional charges.
Note: All proteins are shipped with standard blue ice packs unless otherwise requested. Dry ice shipment requires prior communication and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our standard glycerol concentration is 50% and can serve as a guideline for your own preparation.
Shelf Life
Shelf life depends on various factors, including storage conditions, buffer composition, temperature, and the protein's inherent stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
tilS; PBPRA2951; tRNA(Ile)-lysidine synthase; EC 6.3.4.19; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-435
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Photobacterium profundum (strain SS9)
Target Names
tilS
Target Protein Sequence
MLYSSLRSSL LANIPHSNRF VIALSGGLDS RVLLHLMGRF IQENPQYQCD AVHVHHGLSV
NADKWAQQCL QWAFEEKITC HIEHVTLVLG NRISVEQQAR EQRYLALSKH VQQGDCLLTA
QHADDQLETF LLALKRGSGP AGLASMPEST TFGISYHLRP LLQVTRQSIT DYGIAHQLEW
VEDESNQDQR YDRNFLRHQI TPLLHQRWPG IRKAVSRSAA LCGEQEALLN ELLASHLSKA
LHVDQSLKIV ELGSERIGKQ LIRQWLSLFT VLMPSQAQLQ QIWQSVVLAQ DDANPQVCWD
NHQIRRYKQR LYVVKQWADI SLFQQKCELN QACSLPEGLG TLVLTTVKAN GILRLPQQHE
IVSVRFEPEG IEAKPQGRIG KRKLKKLFQE YEVPSWNRRR TPLVFYNDRL AAVAGLFVTE
DFFGEDCDLD WLCNS
Uniprot No.
Target Background
Function
This enzyme catalyzes the ATP-dependent ligation of lysine to the cytidine at position 34 of the AUA codon-specific tRNA(Ile) containing the CAU anticodon. This modification converts cytidine to lysidine, thereby altering the tRNA's amino acid specificity from methionine to isoleucine.
Database Links
KEGG: ppr:PBPRA2951
STRING: 298386.PBPRA2951
Protein Families
TRNA(Ile)-lysidine synthase family
Subcellular Location
Cytoplasm.
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