Recombinant Photorhabdus luminescens subsp. laumondii 50S ribosomal protein L34 (rpmH)
Description
Introduction
Photorhabdus luminescens subsp. laumondii is a bacterium known for its symbiotic relationship with nematodes and its pathogenic effects on insects . Within the bacterium, the 50S ribosomal protein L34 (rpmH) plays a crucial role in protein synthesis . Recombinant forms of this protein are produced for research, enabling detailed studies of its structure, function, and interactions .
General Information
Production and Characteristics
Recombinant rpmH is produced in various expression systems, including E. coli, yeast, baculovirus, and mammalian cells . The choice of expression system can influence the protein's post-translational modifications and activity. The recombinant protein often includes a tag, such as a His-tag or Avi-tag, to facilitate purification and detection . The purity of the recombinant protein is generally greater than 85%, as determined by SDS-PAGE .
Function and Significance
The 50S ribosomal protein L34 (rpmH) is a component of the large ribosomal subunit and is essential for protein synthesis . Ribosomes are responsible for translating mRNA into proteins, a process vital for cellular function . Specifically, in Photorhabdus laumondii, a toxin inhibits protein synthesis by ADP-ribosylation of the 23S ribosomal RNA, which affects the GTPase activity of elongation factors .
Applications in Research
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Structural Studies: Recombinant rpmH can be used to determine the protein's three-dimensional structure through X-ray crystallography or NMR spectroscopy. This structural information can provide insights into its function and interactions with other ribosomal components.
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Interaction Studies: Recombinant rpmH can be utilized in binding assays to identify its interacting partners. These studies can reveal how rpmH interacts with other ribosomal proteins, RNA molecules, or regulatory factors.
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Functional Assays: Recombinant rpmH can be employed in in vitro translation assays to assess its role in protein synthesis. These assays can measure the impact of rpmH mutations or modifications on translational efficiency and accuracy.
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Antibacterial Toxin Research: Photorhabdus laumondii utilizes a type VI secretion system to deliver toxins that inhibit protein synthesis. Recombinant rpmH can be used to study the effects of these toxins on ribosomal function and structure .
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for custom preparation.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is specifically requested and agreed upon in advance. Additional fees will apply for dry ice shipping.
Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
The specific tag type is determined during production. If you require a particular tag, please inform us, and we will prioritize its development.
