Recombinant Picrophilus torridus 50S ribosomal protein L22P (rpl22p)
Description
Characteristics of Picrophilus torridus
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Genome and Growth Conditions: P. torridus has a genome size of approximately 1,545,900 base pairs with a high coding region percentage of 91.7% and a G+C content of 36% . It is specialized for growth in extremely acidic environments, unable to grow at pH values above 4.0 .
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Metabolic Pathways: The organism relies heavily on a high proton motive force for metabolite transport, lacking Na+-dependent transporters . It also has a significant number of ABC transporters for nutrient uptake, indicating a reliance on peptides and sugars as nutrient sources .
Ribosomal Proteins and Their Functions
Ribosomal proteins are essential components of ribosomes, which are responsible for protein synthesis in cells. While specific studies on rpl22p from P. torridus are not available, ribosomal proteins in general can have extra-ribosomal functions, such as interacting with DNA or participating in cellular processes beyond translation .
Recombinant Protein Expression
Recombinant proteins are expressed in host organisms like Escherichia coli to study their biochemical properties. For example, the glucose/galactose dehydrogenase from P. torridus has been cloned and expressed in E. coli to understand its enzymatic properties . Similarly, recombinant rpl22p would be expressed to study its structure, function, and potential interactions.
Potential Research Directions
Given the lack of specific data on recombinant rpl22p from P. torridus, future research could focus on:
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Expression and Purification: Cloning and expressing rpl22p in a suitable host to study its biochemical properties.
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Structural Analysis: Determining the protein's structure to understand its potential interactions and functions.
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Functional Studies: Investigating any extra-ribosomal functions, such as DNA binding or involvement in stress responses.
Data Table: General Features of Picrophilus torridus Genome
| General Features | Number |
|---|---|
| Genome Size (bp) | 1,545,900 |
| Coding Region (%) | 91.7 |
| G+C Content (%) | 36 |
| Total ORFs | 1,535 |
| ORFs with Assigned Function | 983 |
| ORFs without Function | 553 |
| ORFs without Function or Similarity | 73 |
| ORFs with Putative Signal Peptides | 121 |
| ORFs Involved in Transport | 170 |
| Stable RNAs: rRNAs | 1 5S; 1 16S; 1 23S |
| Stable RNAs: tRNAs | 47 |
Data Table: Biochemical Characteristics of Recombinant Proteins from P. torridus
| Protein | Characteristics |
|---|---|
| Glucose/Galactose Dehydrogenase | Dual cofactor specificity, active with d-galactose and d-glucose |
| NAC Protein | Molecular mass of approximately 14 kDa, exists as monomer and dimer |
References
Product Specs
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The tag type is determined during production. If a specific tag type is required, please inform us, and we will prioritize its incorporation.
Target Background
KEGG: pto:PTO0645
STRING: 263820.PTO0645
