Recombinant Picrophilus torridus Probable porphobilinogen deaminase (hemC)

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Cat. No.
BT2440177
Source
Yeast
Synonyms
hemC; PTO0249; Probable porphobilinogen deaminase; PBG; EC 2.5.1.61; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2440177
Source
E.coli
Synonyms
hemC; PTO0249; Probable porphobilinogen deaminase; PBG; EC 2.5.1.61; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2440177
Source
E.coli
Synonyms
hemC; PTO0249; Probable porphobilinogen deaminase; PBG; EC 2.5.1.61; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2440177
Source
Baculovirus
Synonyms
hemC; PTO0249; Probable porphobilinogen deaminase; PBG; EC 2.5.1.61; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2440177
Source
Mammalian cell
Synonyms
hemC; PTO0249; Probable porphobilinogen deaminase; PBG; EC 2.5.1.61; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase
Purity
>85% (SDS-PAGE)
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Description

Overview of Recombinant Picrophilus torridus Probable Porphobilinogen Deaminase (hemC)

Picrophilus torridus probable porphobilinogen deaminase (PBGD), also known as hydroxymethylbilane synthase (HMBS), is an enzyme involved in the biosynthesis of heme . Heme is an essential molecule for various biological processes, including oxygen transport, electron transfer, and catalysis. PBGD, encoded by the hemC gene, catalyzes the head-to-tail condensation of four porphobilinogen molecules into hydroxymethylbilane, a linear tetrapyrrole precursor of porphyrins .

Reaction Mechanism of Porphobilinogen Deaminase

The enzymatic reaction mechanism involves several steps :

  1. Elimination of ammonia from porphobilinogen to generate a carbocation intermediate.

  2. Attack of the carbocation intermediate by the dipyrrole cofactor, forming a trimer covalently bound to the enzyme.

  3. Repeated addition of porphobilinogen molecules to form a hexamer.

  4. Hydrolysis to release hydroxymethylbilane and regenerate the cofactor.

Relevance to Picrophilus torridus

Picrophilus torridus is an extreme thermoacidophilic archaeon, thriving in highly acidic and high-temperature environments . Its ability to survive in such harsh conditions makes it a subject of interest for understanding the adaptations of life under extreme conditions. The presence of a functional heme biosynthesis pathway, including PBGD, suggests its importance for the organism's survival and metabolic processes .

Product Specs

Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Lead Time
Delivery times vary depending on the purchasing method and location. Please consult your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires advance notification and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, serving as a guideline for your reference.
Shelf Life
Shelf life depends on several factors: storage conditions, buffer components, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing.
The tag type is determined during the production process. If a specific tag type is required, please inform us; we will prioritize its development.
Synonyms
hemC; PTO0249; Probable porphobilinogen deaminase; PBG; EC 2.5.1.61; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-282
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
Target Names
hemC
Target Protein Sequence
MHIRIGTRSS KLAMIQAMMV KDRLDSLGIE TEVKGFTSKG DINLDSPLYS IGGTGVFVDD LNRMILKNEI DIAVHSAKDI PSFIDDSLEI SAVLKRDDPR DVLISQHSLN DLEASSVIGT SSLRRIKELK TLRNDILIKD LRGNIDTRLK KLDNGDYDGI IMAKAAYDRM RINRRHFILN YDDFVPAPNQ GIIAIISKKD SEINDVLKKI NDDETYNDMK AERLILSGLN LGCSKPVGIY AHKNRIFMRF YSLKNDDYKD IVMDYNNIDL EFIRSEIHDY GY
Uniprot No.
Q6L2G8

Target Background

Function
This protein catalyzes the tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen through several distinct steps.
Database Links

KEGG: pto:PTO0249

STRING: 263820.PTO0249

Protein Families
HMBS family

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