Recombinant Pig Neuropeptide Y receptor type 1 (NPY1R)
Description
Expression Systems
| Host System | Purity | Applications | Source |
|---|---|---|---|
| E. coli | ≥85% | SDS-PAGE, WB, Immunogen | |
| Mammalian Cells | ≥85% | Functional assays, Structural studies |
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Tagging: His-tagged variants enable affinity chromatography .
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Yield: Lyophilized powder formulations ensure stability for long-term storage .
Key Research Findings
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Dynamic Behavior: Solid-state NMR and MD simulations reveal conformational changes in apo, agonist-, and arrestin-bound states, critical for ligand binding and signaling .
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Pain Modulation: Y1R knockout mice exhibit heightened sensitivity to thermal/chemical pain, implicating NPY1R in nociceptive pathways .
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Immune Regulation: NPY-Y1R signaling inhibits dendritic cell maturation and promotes Th2 polarization via IL-6/IL-10 .
Pharmacological Profile
| Ligand | Affinity (pM) | Species Tested | Source |
|---|---|---|---|
| Porcine NPY | 43 | Guinea pig, Human | |
| PYY | 48 | Guinea pig | |
| NPY2-36 | 6-fold lower | Guinea pig |
Applications in Research
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Drug Discovery: Used to screen Y1R antagonists (e.g., BIBP3226) for obesity and pain therapies .
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Structural Biology: NMR studies resolve dynamics in lipid membranes, aiding GPCR-targeted drug design .
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Immunology: Elucidates NPY’s role in leukocyte adhesion and granulocyte phagocytosis .
Comparative Evolutionary Insights
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Gene Clustering: Pig NPY1R, NPY2R, and NPY5R cluster on chromosome 8, homologous to human chromosome 4, suggesting conserved evolutionary duplication events .
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Sequence Identity: 92–94% similarity between pig, human, and mouse Y1 receptors .
Key Challenges and Future Directions
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you require a specific format, please indicate your preference in the order notes. We will fulfill your request based on availability.
Note: All protein orders are shipped with standard blue ice packs by default. If you require dry ice shipping, please notify us in advance as additional charges will apply.
Generally, the shelf life of liquid formulations is 6 months at -20°C/-80°C. For lyophilized forms, the shelf life is 12 months at -20°C/-80°C.
