Recombinant Pinus koraiensis Chloroplast Envelope Membrane Protein (cemA) is a protein derived from the chloroplast envelope membrane of Pinus koraiensis, a species of pine tree. This protein is produced through recombinant DNA technology, where the gene encoding the protein is inserted into a host organism, such as Escherichia coli (E. coli), to produce large quantities of the protein. The chloroplast envelope membrane plays a crucial role in the functioning of chloroplasts, which are essential for photosynthesis in plants.
Chloroplast envelope membrane proteins are involved in various critical functions, including:
Ion and Metabolite Transport: These proteins facilitate the movement of ions and metabolites across the chloroplast envelope, which is essential for photosynthesis and other chloroplast functions .
Protein Import Machinery: Proteins in the envelope membrane help in importing proteins synthesized in the cytosol into the chloroplast .
Chloroplast Lipid Metabolism: They are involved in the synthesis and regulation of chloroplast lipids, which are crucial for maintaining membrane structure and function .
| Characteristic | Description |
|---|---|
| Source Organism | Pinus koraiensis (for cemA) |
| Expression Host | Typically Escherichia coli |
| Purity | Often greater than 90% |
| Tag | His-tag for purification and detection |
| Function | Involved in chloroplast envelope membrane functions |