Recombinant Platanus occidentalis Photosystem II CP47 chlorophyll apoprotein (psbB)
Description
Recombinant Production of CP47
Recombinant CP47 is typically expressed in E. coli or mammalian cell systems for structural and functional studies. While P. occidentalis-specific protocols are unreported, methodologies from related species (e.g., Lactuca sativa, Populus deltoides) provide a template :
Table 1: Recombinant CP47 Production Parameters (Generalized)
Notes:
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Tagging: His tags enable affinity chromatography purification .
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Stability: Glycerol (5–50%) is added to prevent aggregation during storage .
Role in PSII Assembly
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CP47 forms a preassembly module (CP47m) with PsbH, PsbL, and PsbT before integrating into the PSII core .
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In Synechocystis sp., Pam68 facilitates chlorophyll insertion into CP47 during membrane integration .
Biotechnological Applications
Recombinant CP47 is pivotal for:
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Photosynthesis Research: Elucidating PSII assembly intermediates and photodamage repair mechanisms .
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Bioengineering: Optimizing light-harvesting efficiency in synthetic photosynthetic systems .
Limitations and Future Directions
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Species-Specific Data: No direct studies on P. occidentalis psbB exist; inferences rely on homologs (e.g., Populus deltoides, UniProt O03061) .
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Functional Validation: In vitro reconstitution assays are needed to confirm chlorophyll-binding kinetics and Mn cluster interactions in recombinant P. occidentalis CP47.
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have a specific format requirement, please indicate it in your order notes, and we will fulfill your request.
Note: All protein shipments include standard blue ice packs. If you require dry ice shipment, please inform us in advance, and additional fees will apply.
Generally, liquid forms have a shelf life of 6 months at -20°C/-80°C. Lyophilized forms typically have a shelf life of 12 months at -20°C/-80°C.
