Recombinant Pongo abelii Membrane-spanning 4-domains subfamily A member 10 (MS4A10)
Description
Structure and Biochemical Characteristics
MS4A10 is a 264-amino-acid protein (Uniprot: Q5REZ6) with a molecular weight of approximately 28–30 kDa. Its structure includes:
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Four transmembrane domains, consistent with the MS4A family’s tetraspanin-like architecture.
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C-terminal cytoplasmic tail critical for interactions with intracellular signaling molecules.
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N-linked glycosylation sites for proper protein folding and stability.
The recombinant protein is often expressed as a partial or full-length construct, depending on the host system. For example, full-length versions (1–264 amino acids) are produced in E. coli or mammalian cells, while partial constructs may exclude non-essential regions .
Production Methods and Purity
Recombinant MS4A10 is synthesized using diverse expression systems:
| Host System | Purity | Key Applications |
|---|---|---|
| E. coli | ≥85% (SDS-PAGE) | Structural studies, binding assays |
| Mammalian Cells (HEK293) | His/Avi/Fc tags | ELISA, functional assays |
| Cell-Free Expression | ≥85% | High-throughput screening |
| Yeast/Baculovirus | ≥85% | Glycoprotein production |
Purity is typically confirmed via SDS-PAGE, and proteins are stored in Tris-based buffers with 50% glycerol at -20°C .
Immune Cell Signaling
MS4A proteins modulate calcium influx and receptor clustering. For example:
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MS4A1 (CD20) regulates B-cell activation and differentiation .
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MS4A2 (FcεRI) amplifies mast cell signaling via FcεRIγ subunit interaction .
Recombinant MS4A10 may serve as a tool to study analogous mechanisms in orangutans.
Alzheimer’s Disease and Neuroinflammation
MS4A4A, a related family member, influences microglial chemokine responses and TREM2 signaling in Alzheimer’s disease (AD) . Recombinant MS4A10 could be used to explore conserved pathways in primates.
Comparative Genomics
MS4A10 is part of the Pongo abelii genome (assembly Susie_PABv2), with orthologs in humans and mice. Comparative studies highlight evolutionary divergence in immune-related genes .
Research Challenges and Gaps
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Limited Functional Studies: Most data derive from related MS4A proteins (e.g., MS4A1, MS4A4A). Direct evidence for MS4A10’s role in Pongo abelii remains sparse.
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Species-Specific Variants: Short tandem repeats (STRs) near MS4A genes may influence population-specific traits, as observed in orangutan subspecies .
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Expression Efficiency: Cell-free or bacterial systems may fail to replicate native glycosylation patterns critical for function .
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes for customized fulfillment.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires advance notification and incurs additional charges.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Target Background
STRING: 9601.ENSPPYP00000003697
UniGene: Pab.7119
