Recombinant Pongo abelii Transmembrane protein 176B (TMEM176B)
Description
Definition and Production
Recombinant Pongo abelii TMEM176B is a 270-amino acid transmembrane protein expressed in E. coli with an N-terminal His tag for purification . Derived from the Sumatran orangutan (Pongo abelii), it corresponds to UniProt ID Q5R8D6 and retains full-length sequence fidelity . Key production parameters include:
Functional Insights
While the recombinant protein itself is primarily a research tool, endogenous TMEM176B regulates:
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Immune Checkpoints: Inhibits NLRP3 inflammasome activation and modulates CD8+/Treg ratios .
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Cancer Pathways: Upregulated in gastric cancer (GC) and linked to tumor size, invasion depth, and lymph node metastasis .
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Fibrosis: Suppresses TGFβ1-SMAD signaling, reducing fibroblast-to-myofibroblast differentiation in pulmonary fibrosis .
Immune Oncology
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TMEM176B knockdown enhances CD8+ T-cell infiltration and synergizes with anti-CTLA-4 therapy in melanoma models .
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Overexpression reduces IL-1β secretion, impairing inflammasome activity in macrophages .
Cancer Studies
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In gastric cancer, high TMEM176B expression correlates with advanced tumor size (≥6 cm; p=0.002), deeper invasion (p=0.021), and lymph node metastasis (p=0.032) .
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Regulates AKT/mTOR signaling in breast cancer, influencing tumor growth and immune evasion .
Fibrosis and Lung Disease
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Adenoviral TMEM176B overexpression in mice attenuates bleomycin-induced pulmonary fibrosis by 40–60%, improving lung compliance and reducing hydroxyproline deposition .
Comparative Analysis with Orthologs
Technical Challenges and Limitations
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Stability Issues: Repeated freeze-thaw cycles degrade the protein; aliquoting in glycerol is critical .
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Species-Specific Variations: Functional disparities between primate and murine orthologs complicate translational research .
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Application Constraints: Primarily validated for SDS-PAGE and ELISA; lacks functional data in live-cell assays .
Future Directions
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Mechanistic Studies: Clarify TMEM176B’s dual role in promoting cancer progression while enhancing antitumor immunity .
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Therapeutic Development: Explore recombinant TMEM176B as a biologic for fibrosis or immune augmentation .
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Structural Biology: Resolve cryo-EM structures to map ion channel domains and ligand-binding sites .
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them in your order. We will fulfill your request to the best of our ability.
Note: All proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance. Additional fees may apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. Lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Target Background
KEGG: pon:100174564
UniGene: Pab.12754
