Recombinant Porphyromonas gingivalis Lipoprotein signal peptidase (lspA)
Description
Functional Role of PG0026 in P. gingivalis
PG0026 is identified as a novel C-terminal signal peptidase responsible for cleaving the C-terminal domain (CTD) signal peptides of virulence factors such as gingipains, which are critical for P. gingivalis pathogenicity . Key features include:
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Catalytic Activity: PG0026 cleaves CTD signals after secretion, enabling protein maturation and surface localization of virulence factors .
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Secretion System: Part of a non-classical Type IX secretion system (T9SS) unique to Bacteroidetes, facilitating the transport of CTD-containing proteins across the outer membrane .
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Genetic Essentiality: Knockout mutants of PG0026 result in defective processing of CTD proteins, impairing bacterial virulence .
Recombinant Applications and Research Findings
Recombinant studies focus on PG0026’s role in virulence and host interactions:
Table 1: Key Findings from PG0026 Studies
Implications for Periodontal Disease
PG0026-processed virulence factors contribute to periodontal pathogenesis by:
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Immune Modulation: Gingipains degrade cytokines (e.g., IL-8) and complement components, impairing neutrophil recruitment .
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Biofilm Formation: PG0026-deficient mutants show altered LPS O-antigen chains, reducing biofilm stability .
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Systemic Effects: P. gingivalis LPS and gingipains promote atherosclerosis via TLR2/4 activation .
Research Gaps and Future Directions
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Structural Studies: High-resolution structures of PG0026 are needed to design inhibitors.
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Recombinant Expression: Limited data exist on recombinant PG0026 production; further optimization could aid functional assays.
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Therapeutic Potential: Targeting PG0026 with monoclonal antibodies or small-molecule inhibitors may disrupt P. gingivalis pathogenicity .
Product Specs
Note: We will prioritize shipping the format we currently have in stock. However, if you require a specific format, please indicate your preference in the order notes. We will accommodate your request whenever possible.
Note: All proteins are shipped with standard blue ice packs. If dry ice shipping is required, please communicate this need in advance, as additional fees will apply.
Generally, liquid forms have a shelf life of 6 months at -20°C/-80°C. Lyophilized forms have a shelf life of 12 months at -20°C/-80°C.
Target Background
KEGG: pgn:PGN_0515
STRING: 431947.PGN_0515
