Recombinant Porphyromonas gingivalis Serine hydroxymethyltransferase (glyA)

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Cat. No.

BT44154

Source

Yeast

Synonyms

glyA; PGN_0038Serine hydroxymethyltransferase; SHMT; Serine methylase; EC 2.1.2.1

Purity

>85% (SDS-PAGE)

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Cat. No.

BT44154

Source

E.coli

Synonyms

glyA; PGN_0038Serine hydroxymethyltransferase; SHMT; Serine methylase; EC 2.1.2.1

Purity

>85% (SDS-PAGE)

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Cat. No.

BT44154

Source

E.coli

Synonyms

glyA; PGN_0038Serine hydroxymethyltransferase; SHMT; Serine methylase; EC 2.1.2.1

Purity

>85% (SDS-PAGE)

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Cat. No.

BT44154

Source

Baculovirus

Synonyms

glyA; PGN_0038Serine hydroxymethyltransferase; SHMT; Serine methylase; EC 2.1.2.1

Purity

>85% (SDS-PAGE)

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Cat. No.

BT44154

Source

Mammalian cell

Synonyms

glyA; PGN_0038Serine hydroxymethyltransferase; SHMT; Serine methylase; EC 2.1.2.1

Purity

>85% (SDS-PAGE)

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Description

Introduction to Serine Hydroxymethyltransferase (SHMT/glyA)

Serine hydroxymethyltransferase (SHMT), encoded by the glyA gene, is a pyridoxal 5'-phosphate (PLP)-dependent enzyme that catalyzes the reversible conversion of serine to glycine while generating 5,10-methylene tetrahydrofolate (MTHF), a key intermediate in one-carbon metabolism. This reaction is critical for purine, thymidylate, and methionine biosynthesis, making SHMT essential for bacterial survival and virulence .

Recombinant SHMT Production and Applications

Although recombinant P. gingivalis SHMT has not been explicitly documented, methodologies for related enzymes provide a framework:

Table 1: Key Steps in Recombinant Protein Production (Inferred from P. gingivalis Systems)

Step	Methodology	Example from P. gingivalis Proteases
Gene Cloning	PCR amplification of glyA ORF with flanking homology regions	Used for rgpA and kgp mutants
Vector Design	Insertion into plasmids (e.g., pQE60) with antibiotic resistance markers	Applied in H. pylori glyA complementation
Expression System	E. coli or yeast-based systems	Yeast systems used for RgpA production
Purification	Affinity chromatography (e.g., His-tag)	Demonstrated for His-tagged RgpB
Validation	Functional assays (e.g., enzymatic activity, complementation)	SHMT activity confirmed via genetic rescue in E. coli ΔglyA

Research Gaps and Future Directions

Structural Insights: No crystal structure of P. gingivalis SHMT exists, though H. pylori SHMT structural studies reveal weak PLP binding and disordered active sites .
Glycosylation: P. gingivalis O-glycosylation modifies periplasmic proteins , but its impact on SHMT remains unexplored.
Therapeutic Targeting: SHMT inhibitors could disrupt folate metabolism, but specific drug design requires further study.

Table 2: SHMT Functional Impact Across Bacterial Species

Organism	SHMT Role	Phenotype of ΔglyA	Relevance to P. gingivalis
H. pylori	MTHF synthesis, glycine auxotrophy	Slow growth, loss of CagA	Suggests SHMT links metabolism to virulence
E. coli	Glycine biosynthesis	Glycine auxotrophy	Highlights conserved metabolic function
P. gingivalis (inferred)	Folate cycle, biofilm symbiosis	Likely impaired growth under nutrient stress	Supported by metabolic cooperativity data

Product Specs

Form

Lyophilized powder. We will preferentially ship the format we have in stock. If you have special format requirements, please note them when ordering.

Lead Time

Delivery times vary by purchase method and location. Consult your local distributor for specific delivery times. All proteins are shipped with normal blue ice packs by default. Request dry ice shipment in advance (extra fees apply).

Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer ingredients, storage temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.

Synonyms

glyA; PGN_0038Serine hydroxymethyltransferase; SHMT; Serine methylase; EC 2.1.2.1

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-426

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561)

Target Names

glyA

Target Protein Sequence

MKKDSVIFDL IEKEHQRQLK GIELIASENF VSEQVMQAMG SCMTNKYAEG YPGKRYYGGC EVVDQSEQIA IDRIKQLYGA EWANVQPHSG AQANMAVLLA CLEAGDTFMG LNLEHGGHLS HGSLVNSSGI LYRPIGYNLS EETGMVDYDH MEKMAIEHKP KLIIGGGSAY SREWDYKRMR EIADKVGALL MIDMAHPAGL IAAGLLENPV KYAHIVTSTT HKTLRGPRGG IILMGKDFDN PWGKKTPKGE IKKMSALLDS AVFPGVQGGP LEHVIAAKAV AFGEALDPSF KEYQTQVKKN AAVLAQAFMD KGYKVISGGT DNHSMLIDLR PKFPELTGKV AEKALVAADI TVNKNMVPFD SRSAFQTSGF RVGTPAITTR GVKEDKMGYI VELIDRVLSA PEDEAVIASV RTEVNRMMAD YPLFAW

Uniprot No.

B2RGR2

Target Background

Function

Catalyzes the reversible interconversion of serine and glycine, using tetrahydrofolate (THF) as the one-carbon carrier. This is the primary source of one-carbon groups for biosynthesis of purines, thymidylate, methionine, and other biomolecules. Also exhibits THF-independent aldolase activity towards beta-hydroxyamino acids, producing glycine and aldehydes via a retro-aldol mechanism.

Database Links

KEGG: pgn:PGN_0038

STRING: 431947.PGN_0038

Protein Families

SHMT family

Subcellular Location

Cytoplasm.

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Recombinant Porphyromonas gingivalis Serine hydroxymethyltransferase (glyA)

Description

Introduction to Serine Hydroxymethyltransferase (SHMT/glyA)

Recombinant SHMT Production and Applications

Table 1: Key Steps in Recombinant Protein Production (Inferred from P. gingivalis Systems)

Research Gaps and Future Directions

Table 2: SHMT Functional Impact Across Bacterial Species

Product Specs

Target Background

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