Recombinant Prochlorococcus marinus PsbF-like protein (PMT9312_1176)
Description
Functional Insights
PMT9312_1176 belongs to the PsbF family, which in cyanobacteria is associated with photosystem II (PSII). While its exact role remains under investigation, functional analogs in PSII include:
Role in Photosynthesis
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PSII Core Complex: PsbF proteins are β-subunits of cytochrome b559, stabilizing the PSII reaction center during light-driven water oxidation .
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Electron Transfer: Belongs to the PsbE/PsbF family, which facilitates proton gradient formation by protecting the reaction center from oxidative damage .
| Interacting Proteins | Function in PSII | Interaction Score |
|---|---|---|
| PsbE | α-subunit of cytochrome b559 | 0.999 |
| PsbD (D2) | Reaction center heterodimer | 0.998 |
| PsbA (D1) | Primary electron donor binding site | 0.997 |
PMT9312_1176’s sequence divergence from canonical PsbF (e.g., in Prochlorococcus strain MIT9301) suggests strain-specific functional adaptations .
Genomic Context and Evolution
PMT9312_1176 is encoded by the PMT9312_1176 gene in the streamlined genome of P. marinus MIT9312, a high-light-adapted strain. Key genomic insights include:
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Gene Amplification: Unlike freshwater cyanobacteria, P. marinus MIT9312 retains a single psbF-like gene, reflecting genomic reductionism .
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Strain-Specific Expression: MIT9312 vesicles lack PMT9312_1176, unlike other vesicle-associated proteins (e.g., PMT9312_0858, 1179) .
Applications in Research
This recombinant protein is utilized in:
Immunological Studies
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ELISA Kits: PMT9312_1176 serves as an antigen for detecting specific antibodies, enabling studies on cyanobacterial protein interactions .
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Biochemical Assays: Purified protein is used to investigate PSII assembly, stability, or ligand binding .
Research Gaps and Future Directions
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Functional Specificity: Direct biochemical assays are needed to confirm PMT9312_1176’s role in PSII versus other processes.
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Interactome Mapping: Partners in DNA repair (e.g., LigW operon proteins) remain unexplored .
References
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Creative BioMart. Recombinant Full Length Prochlorococcus Marinus Psbf-Like Protein (PMT9312_1176).
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PMC. Prochlorococcus Extracellular Vesicles: Molecular Composition and Functional Specificity.
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Afigen. ELISA Recombinant Prochlorococcus marinus PsbF-like Protein.
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Research Commons. DNA Repair Enzymes in Prochlorococcus marinus Strain MIT9312.
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STRING-DB. PsbF Protein (Prochlorococcus marinus MIT9301).
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PMC. Genome Sequence of the Cyanobacterium Prochlorococcus marinus Strain SS120.
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific requirements for the format, please specify them when placing your order. We will prepare the product according to your request.
Note: All of our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please communicate with us in advance. Additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: pmi:PMT9312_1176
STRING: 74546.PMT9312_1176
