Recombinant Prosthecochloris vibrioformis tRNA dimethylallyltransferase (miaA)

Shipped with Ice Packs
In Stock

Product Specs

Form
Lyophilized powder. We will preferentially ship the available format. If you have special format requirements, please note them when ordering.
Lead Time
Delivery times vary by purchase method and location. Consult your local distributor for specifics. All proteins ship with blue ice packs. Dry ice shipping is available upon request for an extra fee.
Notes
Avoid repeated freeze-thaw cycles. Working aliquots are stable at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type is determined during manufacturing. If you require a specific tag, please let us know, and we will prioritize its development.
Synonyms
miaA; Cvib_0776tRNA dimethylallyltransferase; EC 2.5.1.75; Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-309
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Chlorobium phaeovibrioides (strain DSM 265 / 1930) (Prosthecochloris vibrioformis (strain DSM 265))
Target Names
miaA
Target Protein Sequence
MPAKTKEPVA LVITGPTASG KSALAHEVAL RTGAEIISAD SRQIYRELTI GSAKPTPEML GEVPYHFINE KTIGEPYSSG AFATESRARI AMIRKRGNPV IVAGGSPLYL QGLIEGFSDL PPADPKIREK LQKELAEQGS RALYRKLQDL DPERAATLDH TKTHRLIRNL EIIELIGKSS KPPSPTPEMQ FHAVALNFPR EELYQRINLR VEEMMSEGLL QEAEALLKKY RPEISNRTLP ALLTVGYQEL FDHLERKTPL EGAVTLIQQH TRNYAKRQLT FLRNRMQLNW IQAPLTAQER TELAESLLS
Uniprot No.

Target Background

Function
Catalyzes the transfer of a dimethylallyl group to the adenine at position 37 of tRNAs that read codons starting with uridine, forming N6-(dimethylallyl)adenosine (i(6)A).
Database Links
Protein Families
IPP transferase family

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