Recombinant Pseudoalteromonas haloplanktis Glycerol-3-phosphate acyltransferase (plsY)
Description
Biochemical Characteristics
Recombinant Production
Recombinant plsY is typically expressed in E. coli with an N-terminal His-tag for purification. Key specifications include:
| Parameter | Value |
|---|---|
| Source Organism | Pseudoalteromonas haloplanktis TAC125 (strain TAC 125) |
| Expression Host | E. coli |
| Protein Length | 1–203 amino acids (full-length) |
| Purity | >90% (SDS-PAGE analysis) |
| Storage Buffer | Tris-based buffer, 50% glycerol, pH 8.0 |
| Storage Conditions | -20°C/-80°C (avoid repeated freeze-thaw cycles) |
| Reconstitution | Deionized sterile water (0.1–1.0 mg/mL), with optional glycerol addition |
Functional Role
PlsY operates in the PlsX-PlsY pathway, converting acyl-acyl carrier protein (ACP) to acyl-phosphate via PlsX, followed by acyl transfer to glycerol-3-phosphate. This reaction is essential for initiating membrane phospholipid biosynthesis in bacteria .
Research Applications
Recombinant Protein Production
While P. haloplanktis TAC125 is engineered as a psychrophilic host for challenging proteins , plsY itself is produced in E. coli due to its compatibility with mesophilic expression systems. Recent advances in P. haloplanktis plasmid engineering (e.g., high-copy OriR derivatives) aim to enhance recombinant yield, though plsY production remains E. coli-centric .
Role in Lipid Biosynthesis
PlsY’s activity is pivotal for membrane lipid homeostasis, particularly in cold-adapted bacteria. Its psychrophilic adaptations enable efficient catalysis at low temperatures, a trait under investigation for biotechnological applications .
Comparative Analysis of PlsY Orthologs
Future Directions
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Cold-Adapted Catalysis: Structural studies to elucidate temperature-dependent conformational changes.
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Plasmid Engineering: Potential re-introduction of plsY into P. haloplanktis for dual-host systems (e.g., psychrophilic and mesophilic).
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Metabolic Engineering: Leveraging plsY to modulate phospholipid composition in biofilm-forming bacteria .
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them during order placement. We will fulfill your request if possible.
Note: All our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance as additional charges will apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: pha:PSHAa2303
STRING: 326442.PSHAa2303
