Recombinant Pseudomonas entomophila UPF0114 protein PSEEN0819 (PSEEN0819)
Description
Introduction to Recombinant Pseudomonas entomophila UPF0114 protein PSEEN0819 (PSEEN0819)
Recombinant Pseudomonas entomophila UPF0114 protein PSEEN0819 (PSEEN0819) is a protein derived from the bacterium Pseudomonas entomophila. Proteins are fundamental components of biological systems, crucial in various biological processes . The primary structure of a protein refers to its amino acid sequence, which dictates its shape and function1.
Primary, Secondary, Tertiary, and Quaternary Structures of Proteins
Proteins have four levels of structural organization: primary, secondary, tertiary, and quaternary .
Pseudomonas entomophila and the PSEEN0819 Protein
Pseudomonas entomophila is a bacterium known for its insecticidal properties . Proteins such as PSEEN0819, derived from P. entomophila, may play a role in its interaction with insects.
Recombinant Production of PSEEN0819
Recombinant proteins are produced using genetic engineering techniques, where the gene encoding the protein of interest is inserted into a host organism (e.g., E. coli) that then produces the protein . Recombinant PSEEN0819 protein is often used in research and development.
Applications of Recombinant PSEEN0819
Recombinant PSEEN0819 has uses in:
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ELISA Assays: It can be employed as an antigen in Enzyme-Linked Immunosorbent Assays (ELISA) . ELISA is a biochemical technique used to detect the presence of an antibody or an antigen in a sample.
Presenilins and γ-Secretase
Presenilins are key components of the γ-secretase complex, an intramembrane protease crucial in various cellular processes .
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Structure of Presenilins: Presenilins, such as PSEN1 and PSEN2, are integral membrane proteins with multiple transmembrane domains .
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Function in γ-Secretase Activity: Presenilins are catalytic subunits of γ-secretase, which cleaves other proteins within their transmembrane domains . Mutations in presenilin genes can affect γ-secretase activity and are implicated in diseases such as Alzheimer's .
The Dystrophin Complex
The dystrophin complex is vital in stabilizing the plasma membrane of muscle cells . Dystrophin, a key component, has four main functional domains:
Product Specs
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The tag type is determined during production. If you require a specific tag, please inform us; we will prioritize its development.
Target Background
KEGG: pen:PSEEN0819
STRING: 384676.PSEEN0819
Q&A
Basic Research Questions
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How should recombinant PSEEN0819 be properly stored and handled in laboratory settings?
For optimal results when working with recombinant PSEEN0819, researchers should follow these evidence-based protocols:
Storage Parameter Recommended Condition Notes Long-term storage -20°C to -80°C Aliquoting necessary for multiple use Working aliquots 4°C Stable for up to one week Buffer composition Tris/PBS-based, 6% Trehalose, pH 8.0 Optimized for stability Reconstitution Deionized sterile water (0.1-1.0 mg/mL) Centrifuge vial before opening Cryoprotectant 5-50% glycerol (final concentration) 50% is standard reference Repeated freeze-thaw cycles should be strictly avoided as they significantly reduce protein activity . For experimental reproducibility, consistent handling practices should be maintained across studies.
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What expression systems are used for producing recombinant PSEEN0819?
Recombinant PSEEN0819 is predominantly produced using E. coli expression systems. The standard methodology involves:
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Cloning the full-length gene (nucleotides encoding amino acids 1-162) into an expression vector
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Adding an N-terminal His-tag for purification purposes
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Transforming the construct into E. coli expression strains
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Inducing protein expression under optimized conditions
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Purifying via affinity chromatography targeting the His-tag
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Confirming purity (>90%) using SDS-PAGE analysis
This approach yields recombinant protein suitable for functional studies, antibody production, and structural analyses. Similar methodologies are employed for homologous proteins from P. putida (PputGB1_0714) .
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