Recombinant Pseudomonas stutzeri Membrane protein nosY
Description
Introduction to Recombinant Pseudomonas stutzeri Membrane Protein nosY
Recombinant Pseudomonas stutzeri Membrane Protein nosY (UniProt ID: P19845) is a full-length integral membrane protein (1-276 amino acids) encoded by the nosY gene. It functions as a permease component of an ATP-binding cassette (ABC) transporter system (nosDFY) critical for copper handling and nitrous oxide reductase (NOR) maturation in denitrifying bacteria . The recombinant form is typically expressed in E. coli or Pseudomonas stutzeri with an N-terminal His-tag for purification and structural studies .
Primary Structure
nosY exhibits a hydrophobic transmembrane domain structure typical of ABC transporter permeases. Its amino acid sequence (MW ~30 kDa) includes motifs for membrane integration and ATP-independent substrate translocation .
Functional Role
nosY operates as part of the nosDFY complex, which lacks canonical ATPase subunits but uses ATP hydrolysis to facilitate copper transfer from the chaperone NosL to nitrous oxide reductase (NOR) . Structural cryo-EM studies reveal that nosY stabilizes the periplasmic domain of NosD, enabling conformational changes critical for copper delivery .
Expression Systems
Functional Studies
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them when placing your order. We will accommodate your request whenever possible.
Note: All protein shipments are sent with standard blue ice packs. If dry ice is required, please communicate this need in advance as additional fees will apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
