Recombinant Pseudomonas syringae pv. tomato Outer-membrane lipoprotein carrier protein (lolA)

Shipped with Ice Packs
In Stock

Product Specs

Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Lead Time
Delivery times vary depending on the purchase method and location. Please consult your local distributor for precise delivery estimates.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires advance notification and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50% and can serve as a reference.
Shelf Life
Shelf life depends on storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses to prevent repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing.
The tag type is determined during production. If you require a specific tag, please inform us; we will prioritize its development.
Synonyms
lolA; PSPTO_3348; Outer-membrane lipoprotein carrier protein
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
22-207
Protein Length
Full Length of Mature Protein
Purity
>85% (SDS-PAGE)
Species
Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Target Names
lolA
Target Protein Sequence
DGKDVARLT QLLEKSQTLT ARFSQLTLDG GGTQLQETTG EMALQRPGLF NWHTDAPQEQ LMVSDGKKVS LWDPDLEQVT IKKLDQRLTQ TPALLLSGDV SKISESFDIT AKEAGGVIDF MLKPKTKDTL FDSLRLSFRN GIINDMQLID SVGQRTNILF TGVKANESIA ASKFQFQIPK GADVIQE
Uniprot No.

Target Background

Function

This protein participates in the translocation of lipoproteins from the inner membrane to the outer membrane. Complex formation with a lipoprotein only occurs if the residue following the N-terminal cysteine is not an aspartate. Aspartate acts as a targeting signal, retaining the lipoprotein in the inner membrane.

Database Links
Protein Families
LolA family
Subcellular Location
Periplasm.

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