Recombinant Pseudomonas syringae pv. tomato tRNA (uracil (54)-C (5))-methyltransferase (trmA)
Description
Definition and Classification
tRNA (uracil (54)-C(5))-methyltransferase (trmA) is an enzymatic protein responsible for methylating the C(5) position of uridine at position 54 (m⁵U54) in bacterial transfer RNA (tRNA). This modification is critical for tRNA stability, codon-anticodon recognition, and translation fidelity . The recombinant form of this enzyme from Pseudomonas syringae pv. tomato (strain DC3000) is commercially available and characterized for structural and functional studies .
Functional Role
trmA catalyzes the methylation of uridine-54 in tRNA, a conserved modification across bacteria. This reaction ensures proper tRNA structure, enhances ribosome binding during translation, and prevents frameshifting or misincorporation of amino acids .
In P. syringae pv. tomato, trmA may contribute to:
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Pathogenicity: Efficient protein synthesis during host invasion.
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Stress Adaptation: Maintenance of translational accuracy under adverse conditions.
Functional Specificity
Studies in Pyrococcus abyssi revealed that homologs of trmA (e.g., PAB0719) exhibit TrmA-like activity, methylating uridine-54 in tRNA rather than rRNA. This functional shift highlights evolutionary flexibility in methyltransferase substrate specificity .
Horizontal Gene Transfer
Phylogenetic analysis suggests that trmA-like genes in archaea (e.g., Thermococcales) were acquired via horizontal transfer from bacterial donors. This transfer enabled functional adaptation to methylate tRNA instead of rRNA .
Applications and Experimental Utility
The recombinant trmA from P. syringae pv. tomato is used in:
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Structural Biology: Crystallization studies to elucidate catalytic mechanisms.
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Biochemical Assays: Enzyme activity tests using synthetic tRNA substrates.
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Comparative Genomics: Evolutionary studies of tRNA modification systems.
Comparative Analysis: trmA vs. RumA
| Feature | trmA (bacterial) | RumA (bacterial) |
|---|---|---|
| Substrate | tRNA (uridine-54) | 23S rRNA (m⁵U1939) |
| SAM dependence | Yes | Yes |
| Functional role | tRNA stability, translation | rRNA maturation, ribosome assembly |
| Example organisms | P. syringae, E. coli | E. coli, P. abyssi |
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify any format requirements in your order notes for customized preparation.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Target Background
KEGG: pst:PSPTO_4654
STRING: 223283.PSPTO_4654
