Recombinant Pseudomonas syringae pv. tomato Tryptophan--tRNA ligase (trpS)
Description
Introduction
Pseudomonas syringae pv. tomato is a bacterial pathogen that affects tomato plants, causing diseases such as bacterial speck . Pseudomonas syringae strains use a type III secretion system (TTSS) to inject effector proteins into host cells, suppressing the plant's immune system and facilitating pathogenesis . Among the various components involved in the pathogenicity of P. syringae, Tryptophan--tRNA ligase (TrpS) plays a crucial role. TrpS, encoded by the trpS gene, is responsible for catalyzing the attachment of tryptophan to tRNA^{Trp}\, a necessary step for protein synthesis .
Tryptophan--tRNA Ligase (TrpS) Function
Tryptophan--tRNA ligase (TrpS) is an enzyme that belongs to the aminoacyl-tRNA ligase family. Its primary function is to ensure the accurate translation of genetic information by attaching tryptophan to its corresponding tRNA molecule . This process is essential for incorporating tryptophan into proteins during protein synthesis.
Role in Pathogenicity
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General Virulence Factors\
A newly discovered gene, TvrR, is required for virulence of P. syringae pv. tomato strain DC3000 on Arabidopsis thaliana and tomato plants . It encodes a TetR family transcriptional regulator, influencing virulence in DC3000 . -
1-Methyltryptophan (1-MT) Effects\
1-MT protects tomato plants against P. syringae and B. cinerea . It reduces disease symptoms and the number of colony-forming units (cfu) after treatment . 1-MT modifies the apoplast content in tomato plants, enhancing resistance against P. syringae . It also affects bacterial motility, reducing the capacity for infection . -
HrpZ Gene\
The hrpZ gene in Pseudomonas syringae pv. maculicola M2 is necessary for pathogenicity . Mutants with transposon insertion in hrpZ are unable to infect Arabidopsis thaliana or Brassica oleracea plants .
Research Findings
Research has provided insights into the role of TrpS and related factors in the pathogenicity of P. syringae pv. tomato.
Molecular Interactions and Regulation
P. syringae employs a range of effectors and regulatory mechanisms to modulate plant defense responses and facilitate infection. HopPtoD2, a translocated effector with protein tyrosine phosphatase activity, modulates plant defense responses . AvrPtoB suppresses the plant's innate immune system through its multiple domains and protein interactions . The production of coronatine by P. syringae also plays a role, mimicking wound responses and triggering JA-regulated genes .
Product Specs
Note: We will ship the available format preferentially. If you require a specific format, please specify this in your order notes.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The specific tag type is determined during production. If you require a particular tag, please inform us, and we will prioritize its development.
Target Background
Catalyzes the attachment of tryptophan to tRNA(Trp).
KEGG: pst:PSPTO_4429
STRING: 223283.PSPTO_4429
