RPmUP1 and related isoforms are primarily used in:
Genetic Mapping:
Linkage analysis for adaptive traits (e.g., drought resistance, wood quality).
Marker development for population genetics (Table 1).
Functional Genomics:
Identification of candidate genes under selection (e.g., F3H1, MT-like).
Association studies for phenotypic traits like disease resistance.
Protein Interaction Studies:
Role in signaling pathways (e.g., SA-dependent NPR1 interactions) inferred from Arabidopsis homologs.
Table 1: Polymorphism Metrics for Douglas-Fir Candidate Genes
Gene
Total Haplotypes
π (Nucleotide Diversity)
Θ<sub>W</sub> (Watterson’s Estimator)
Selection Signal
EF1A
17
0.00274 ± 0.00020
0.00339 ± 0.00136
Neutral
MT-like
13
0.01334 ± 0.00098
0.00911 ± 0.00342
Positive
F3H1
7
0.00528 ± 0.00179
0.00988 ± 0.00396
Negative
Data derived from 18 candidate genes analyzed for nucleotide diversity and selection.
Comparative Genomic Insights
Population Genetics:
Douglas-fir exhibits high SNP diversity (π = 0.00655, Θ<sub>W</sub> = 0.00702) compared to other conifers like loblolly pine (π = 0.0041).
Linkage Disequilibrium (LD):
Rapid LD decay (~50% within 2,000 bp) enables precise association mapping.
Functional Hypotheses
RPmUP1 may contribute to:
Stress Adaptation: Analogous to MT-like and LEA2 genes involved in drought response.
Defense Signaling: Potential overlap with SA/NPR1 pathways regulating pathogen resistance.
Research Gaps and Future Directions
Functional Characterization: Targeted knockout/overexpression studies in model systems.
Structural Biology: Cryo-EM or X-ray crystallography to resolve 3D conformation.
Ecological Studies: Field trials to assess phenotypic impacts under climate stress.
Product Specs
Form
Lyophilized powder. We will ship the format we have in stock. If you have special format requirements, please note them when ordering, and we will try to accommodate your request.
Lead Time
Delivery times vary depending on purchasing method and location. Please consult your local distributors for specific delivery times. All proteins are shipped with standard blue ice packs. For dry ice shipping, please contact us in advance; extra fees apply.
Notes
Avoid repeated freezing and thawing. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50% for your reference.
Shelf Life
Shelf life depends on several factors, including storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you require a specific tag type, please inform us, and we will prioritize developing it.