Recombinant Psilotum nudum NAD (P)H-quinone oxidoreductase subunit 3, chloroplastic (ndhC)
Description
Overview of Recombinant Psilotum nudum NAD(P)H-quinone Oxidoreductase Subunit 3, Chloroplastic (ndhC)
Recombinant Psilotum nudum NAD(P)H-quinone oxidoreductase subunit 3, chloroplastic (ndhC) is a protein involved in the electron transport chain within the chloroplasts of plants, specifically in Psilotum nudum, also known as the whisk fern . This protein is a subunit of the NAD(P)H dehydrogenase complex, which plays a role in various photosynthetic processes .
Function and Role
The primary function of the ndhC subunit is to shuttle electrons from NAD(P)H to plastoquinone, utilizing FMN and iron-sulfur centers to facilitate this transfer . This process is crucial for:
Association with Chloroplast Lipid Droplets
The NAD(P)H dehydrogenase C1 (NDC1) is found to associate with plastoglobules, a type of chloroplast lipid droplet . NDC1 reduces a plastoquinone analog in vitro and influences the redox state of the plastoquinone pool by reducing the plastoquinone reservoir of plastoglobules .
Experimental Evidences
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In Vitro Activity: Recombinant NDC1 can utilize decyl-PQ as a substrate with NADPH as the electron donor . Purified plastoglobules can function as a quinone-containing substrate, accepting electrons from NADPH and recombinant NDC1 in vitro .
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In Vivo Impact: Mutants lacking NDC1 exhibit a more oxidized plastoquinone pool compared to wild-type plants . NDC1 is essential for normal plastochromanol-8 accumulation and vitamin K1 production .
Potential Applications
Understanding the function and structure of ndhC can have implications for:
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Crop Improvement: Enhancing photosynthetic efficiency and stress tolerance in plants .
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Biotechnology: Engineering plants for improved production of valuable compounds such as vitamins and antioxidants .
Data Table
Future Research Directions
Further research could focus on:
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Detailed Structural Analysis: High-resolution structural studies to understand the precise mechanism of electron transfer.
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Regulation: Investigating the regulatory mechanisms that control the expression and activity of ndhC.
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Interaction with Other Proteins: Identifying other proteins that interact with ndhC to form the functional NAD(P)H dehydrogenase complex .
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Role in Stress Response: Understanding the role of ndhC in plant responses to environmental stresses such as high light and drought.
Product Specs
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Target Background
Function: NDH (NAD(P)H-quinone oxidoreductase) shuttles electrons from NAD(P)H:plastoquinone, utilizing FMN and iron-sulfur (Fe-S) centers, to quinones within the photosynthetic electron transport chain and potentially a chloroplast respiratory chain. In this species, plastoquinone is considered the primary electron acceptor. The enzyme couples this redox reaction to proton translocation, thus conserving redox energy as a proton gradient.
