Recombinant Putative membrane protein mmpS4 (ML2377)
Description
Introduction to Recombinant Putative Membrane Protein MmpS4 (ML2377)
Recombinant Putative membrane protein MmpS4 (ML2377) is a protein derived from Mycobacterium leprae, a bacterium responsible for leprosy. This protein is part of a siderophore export system crucial for the virulence of mycobacteria, including Mycobacterium tuberculosis (M. tuberculosis), which causes tuberculosis. The recombinant form of MmpS4 is produced in yeast and is used for research purposes, particularly in studying the mechanisms of iron acquisition and siderophore secretion in mycobacteria.
Function and Role of MmpS4
MmpS4 plays a critical role in the export of siderophores, which are iron-chelating compounds essential for the survival of mycobacteria within their host. It interacts with transporter proteins like MmpL4 and MmpL5 to facilitate the secretion of these siderophores. This interaction is crucial for the virulence of M. tuberculosis, as mutants lacking MmpS4 and its associated transporters exhibit significantly reduced virulence in animal models .
Characteristics of Recombinant MmpS4
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Source: The recombinant MmpS4 protein is produced in yeast .
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Purity: The protein has a purity of more than 85% as determined by SDS-PAGE .
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Storage: It is recommended to store the protein in a lyophilized form at -20°C or -80°C for up to 12 months. Liquid forms have a shelf life of 6 months at these temperatures .
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Reconstitution: The protein should be reconstituted in deionized sterile water to a concentration of 0.1-1.0 mg/mL, with the addition of 5-50% glycerol for long-term storage .
Interaction with Transporter Proteins
MmpS4 binds to the periplasmic loop of MmpL4 and also interacts with MmpL5, forming part of a novel siderophore export system in mycobacteria . This interaction is essential for the efficient biosynthesis and secretion of siderophores.
Impact on Virulence
Deletion of the mmpS4 and mmpS5 genes in M. tuberculosis results in a significant reduction in bacterial virulence. The bacterial burden in mouse lungs decreases by 10,000-fold, and none of the infected mice die within 180 days, compared to wild-type M. tuberculosis .
Table 1: Characteristics of Recombinant MmpS4
| Characteristic | Description |
|---|---|
| Source | Yeast |
| Purity | >85% (SDS-PAGE) |
| Storage | Lyophilized: 12 months at -20°C/-80°C; Liquid: 6 months at -20°C/-80°C |
| Reconstitution | 0.1-1.0 mg/mL in deionized sterile water with 5-50% glycerol |
Table 2: Impact of MmpS4 Deletion on Virulence
| Strain | Virulence in Mice |
|---|---|
| Wild-type M. tuberculosis | High virulence |
| ΔmmpS4/S5 mutant | Significantly reduced virulence |
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes if different. We will accommodate your request whenever possible.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping is available upon request with an additional charge; please contact us in advance to arrange this.
Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
The specific tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its inclusion.
Target Background
KEGG: mle:ML2377
STRING: 272631.ML2377
