Recombinant Putative S-adenosyl-L-methionine-dependent methyltransferase Mb3793c (Mb3793c)
Description
Introduction to Recombinant Putative S-adenosyl-L-methionine-dependent methyltransferase Mb3793c (Mb3793c)
Recombinant Putative S-adenosyl-L-methionine-dependent methyltransferase Mb3793c (Mb3793c) is an enzyme that catalyzes a chemical reaction utilizing S-adenosyl-L-methionine . Methyltransferases, like Mb3793c, are crucial in many biological processes, as they transfer a methyl group from a donor molecule, typically S-adenosyl-L-methionine (SAM), to an acceptor molecule . These enzymes play significant roles in DNA methylation, RNA methylation, protein methylation, and the biosynthesis of various metabolites .
Function and Mechanism
Mb3793c is classified as a methyltransferase, which means it facilitates the transfer of a methyl group from S-adenosyl-L-methionine to a specific substrate . The general reaction catalyzed by methyltransferases is:
$$
S-adenosyl-L-methionine + acceptor \rightarrow S-adenosyl-L-homocysteine + methylated : acceptor
$$
The enzyme binds to both S-adenosyl-L-methionine and the target substrate. The methyl group from S-adenosyl-L-methionine is then transferred to the substrate, resulting in S-adenosylhomocysteine and the methylated product .
Role in RNA Methylation and Disease
Mutations in RNA methylating enzymes can result in diseases and disorders . For example, NSun3-mediated methylation in mitochondrial tRNA is essential for mitochondrial protein synthesis, and loss-of-function mutations in NSun3 can lead to mitochondrial respiratory chain disorder . Similarly, mutations in METTL3 and METTL14, components of the m6A methyltransferase complex, have been identified in cancer patients, affecting methylation and RNA substrate specificity .
Tables Illustrating Key Information
Tables are used to organize detailed data, allowing readers to quickly understand results . The title should clearly describe the table's content, and column heads should indicate the nature of the data presented .
| Enzyme | Substrate | Reaction Catalyzed | Biological Role | Disease Implication |
|---|---|---|---|---|
| Mb3793c | S-adenosyl-L-methionine | Methyl group transfer to a specific substrate | Biosynthesis of metabolites, regulation of gene expression | (Further research needed to establish specific links) |
| METTL3 | RNA | m6A methylation | mRNA stability and translation | Colon cancer (Tyr406Cys mutation) |
| METTL14 | RNA | Positions RNA for methylation by METTL3 | mRNA stability and translation | Endometrial cancer (Arg298Pro mutation) |
| NSun3 | tRNA | Methylation at the wobble cytosine position in mitochondrial tRNA | Mitochondrial protein synthesis | Mitochondrial respiratory chain disorder |
Product Specs
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Target Background
Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity.
