Recombinant Pyrenophora tritici-repentis Cytoplasmic tRNA 2-thiolation protein 2 (ncs2)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder. Note: We will preferentially ship the format we have in stock. If you have special format requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery time may vary depending on the purchase method and location. Please consult your local distributor for specific delivery times. Note: All proteins are shipped with standard blue ice packs by default. If you require dry ice shipping, please contact us in advance, as additional charges will apply.
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect the contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50% for your reference.
Shelf Life
Shelf life depends on several factors, including storage conditions, buffer components, storage temperature, and protein stability. Generally, the shelf life for the liquid form is 6 months at -20°C/-80°C, and for the lyophilized form, it is 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you have a specific tag type requirement, please inform us, and we will prioritize developing the specified tag.
Synonyms
ncs2; ctu2; PTRG_05556; Cytoplasmic tRNA 2-thiolation protein 2
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-380
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Pyrenophora tritici-repentis (strain Pt-1C-BFP) (Wheat tan spot fungus) (Drechslera tritici-repentis)
Target Names
ncs2
Target Protein Sequence
MPGKEIVDAS NEPCRRCKAS SVLVVRSEPL CHDCFARYIH TKCIKRLETF RVNFTAEQQP
RVLVPLSFGI SSVTLLHVLD LHQRTQKSKT GRTGFNISAV YIEDPEQSIM AEDLLDQVRE
QYSGHQYASL PLHDVFRLVQ DDASIRSLVP ETQYASTPEE KLVHMINSLT SATARADVLS
TLKTRLIVEH AKLTGCESIL WGDSTTRLAQ KTLSETAKGR GFSLPWQVSD GPSPFGLNFH
YPLRDVLKKE LVSYMNMAET GLKTLVHETS IGATQASTSS KNTTIDDLMK QYFESVEDNF
PSIVANVVRT ASRLEAQPDA LSEPKCSLCS MPVSGGRFGI HGWGGDQQDG DDFGSTHANR
TICYGCTRSV PKATSSTNGN
Uniprot No.
Target Background
Function
Plays a central role in the 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu), and tRNA(Gln). It likely functions by forming a heterodimer with ncs6, which transfers sulfur from thiocarboxylated urm1 onto the uridine of tRNAs at the wobble position. Prior mcm(5) tRNA modification by the elongator complex is necessary for 2-thiolation. May also be involved in protein urmylation.
Database Links
STRING: 426418.XP_001935889.1
Protein Families
CTU2/NCS2 family
Subcellular Location
Cytoplasm.
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