Recombinant Ralstonia metallidurans 4-hydroxybenzoate octaprenyltransferase (ubiA)
Description
Introduction to Recombinant Ralstonia metallidurans 4-Hydroxybenzoate Octaprenyltransferase (ubiA)
Recombinant Ralstonia metallidurans 4-hydroxybenzoate octaprenyltransferase, encoded by the gene ubiA, is a crucial enzyme involved in the biosynthesis of ubiquinone, a vital component in the electron transport chain of bacteria. This enzyme catalyzes the transfer of an octaprenyl group to 4-hydroxybenzoate, a key step in the synthesis of ubiquinone. The recombinant form of this enzyme is produced in Escherichia coli and is used for various biochemical studies and applications.
Characteristics of Recombinant Ralstonia metallidurans 4-Hydroxybenzoate Octaprenyltransferase (ubiA)
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Source and Production: The recombinant enzyme is produced in E. coli, using the genetic material from Ralstonia metallidurans (previously known as Cupriavidus metallidurans) .
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Purity and Stability: The enzyme is purified to a level of >85% as determined by SDS-PAGE. It is stable when stored at -20°C or -80°C, with a shelf life of 6 months in liquid form and 12 months in lyophilized form .
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Function: This enzyme is essential for the biosynthesis of ubiquinone by transferring an octaprenyl group to 4-hydroxybenzoate.
Biochemical Properties
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Optimal Conditions: While specific optimal conditions for the recombinant Ralstonia metallidurans enzyme are not detailed, related enzymes like those from E. coli are known to be optimal at pH 7.8 and require magnesium ions for activity .
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Substrate Specificity: The enzyme typically accepts all-trans-octaprenyl diphosphate as the prenyl donor, although specific Km values for this recombinant form are not provided.
Research Findings and Applications
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Ubiquinone Biosynthesis: The enzyme plays a critical role in the synthesis of ubiquinone, which is essential for bacterial energy metabolism and electron transport .
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Biotechnological Applications: Recombinant enzymes like this can be used in biotechnological applications, such as the production of ubiquinone analogs or in studies related to bacterial metabolism and energy production.
Table 1: Characteristics of Recombinant Ralstonia metallidurans 4-Hydroxybenzoate Octaprenyltransferase (ubiA)
| Characteristic | Description |
|---|---|
| Source | E. coli |
| Purity | >85% (SDS-PAGE) |
| Storage Conditions | -20°C or -80°C |
| Shelf Life | 6 months (liquid), 12 months (lyophilized) |
| Function | Ubiquinone biosynthesis |
Recombinant Ralstonia metallidurans 4-hydroxybenzoate octaprenyltransferase (ubiA) is a valuable tool for studying ubiquinone biosynthesis and has potential applications in biotechnology. Further research into its biochemical properties and applications could enhance our understanding of bacterial metabolism and energy production pathways.
Product Specs
Note: We will prioritize shipping the format we currently have in stock. However, if you require a specific format, please specify your preference when placing the order. We will accommodate your request to the best of our ability.
Note: All our proteins are shipped with standard blue ice packs by default. If you require dry ice shipping, please inform us in advance as additional fees will apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: rme:Rmet_2939
STRING: 266264.Rmet_2939
