Recombinant Rat 2-oxoglutarate receptor 1 (Oxgr1)
Description
Functional Roles and Research Applications
Recombinant Oxgr1 is utilized to study its role in:
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Ligand Binding: αKG and itaconate activate Oxgr1 at millimolar concentrations, triggering intracellular Ca²⁺ signaling and Gq/11-mediated pathways .
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Cysteinyl Leukotriene Interactions: LTE4 binding to Oxgr1 regulates airway inflammation and brush cell proliferation in respiratory tissues .
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Metabolic Regulation: Oxgr1 modulates glucose and lipid metabolism in skeletal muscle, influencing capillary density and fiber-type composition .
Cardiac Hypertrophy
Oxgr1 ablation in mice exacerbates pressure overload-induced hypertrophy, while its overexpression reduces cardiomyocyte enlargement. Mechanistically, Oxgr1 interacts with CSN5 (COP9 subunit 5) to suppress STAT3 signaling, a pro-hypertrophic pathway .
Renal Function
In diabetic models, Oxgr1 mediates αKG-induced upregulation of the (pro)renin receptor (PRR) and GLUT1 in collecting duct cells, contributing to sodium retention and angiotensin II formation .
Skeletal Muscle Metabolism
Oxgr1 knockout mice exhibit reduced muscle mass, capillary density, and oxidative fiber content. Recombinant Oxgr1 may be used to study its role in vascular smooth muscle relaxation and metabolic adaptation .
Methodological Insights
Recombinant Oxgr1 is validated for:
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ELISA/Western Blotting: Antibodies like A12225 (Boster Bio) detect Oxgr1 in rat tissues and recombinant proteins .
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Functional Assays: αKG-induced Ca²⁺ influx and ERK phosphorylation are quantified in Xenopus oocytes expressing Oxgr1 .
Clinical and Therapeutic Implications
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Target Validation: Recombinant Oxgr1 aids in identifying antagonists (e.g., ML) to inhibit pathways linked to asthma, diabetes, or cardiac disease .
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Drug Development: Structural studies using recombinant Oxgr1 inform the design of small-molecule modulators targeting its hydrophobic pocket (e.g., ARG110, HIS114 residues) .
Product Specs
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Target Background
- P2Y15 is a cell surface receptor for AMP and adenosine. PMID: 15001573
