Recombinant Rat Histone-binding protein RBBP7 (Rbbp7)

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Cat. No.
BT2484272
Source
Yeast
Synonyms
Rbbp7; Histone-binding protein RBBP7; Nucleosome-remodeling factor subunit RBAP46; Retinoblastoma-binding protein 7; RBBP-7
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2484272
Source
E.coli
Synonyms
Rbbp7; Histone-binding protein RBBP7; Nucleosome-remodeling factor subunit RBAP46; Retinoblastoma-binding protein 7; RBBP-7
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2484272
Source
E.coli
Synonyms
Rbbp7; Histone-binding protein RBBP7; Nucleosome-remodeling factor subunit RBAP46; Retinoblastoma-binding protein 7; RBBP-7
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2484272
Source
Baculovirus
Synonyms
Rbbp7; Histone-binding protein RBBP7; Nucleosome-remodeling factor subunit RBAP46; Retinoblastoma-binding protein 7; RBBP-7
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2484272
Source
Mammalian cell
Synonyms
Rbbp7; Histone-binding protein RBBP7; Nucleosome-remodeling factor subunit RBAP46; Retinoblastoma-binding protein 7; RBBP-7
Purity
>85% (SDS-PAGE)
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Description

Overview of Recombinant Rat Histone-Binding Protein RBBP7 (Rbbp7)

Recombinant Rat Histone-Binding Protein RBBP7 (Rbbp7) is a protein that belongs to a highly conserved subfamily of WD-repeat proteins and is a nuclear protein expressed ubiquitously . RBBP7 is a core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases, and histone deacetylases to their histone substrates in a manner regulated by nucleosomal DNA . RBBP7 is similar to RBBP4, and these proteins are crucial histone chaperones that govern the molecular behavior of histones H3 and H4 .

Function and Molecular Mechanisms

RBBP7 is involved in several crucial functions:

  • Chromatin Remodeling: RBBP4/7 can influence histone conformation through direct binding to histone H3 and H4 . As part of the NuRD complex, RBBP4/7 can alter chromatin structure, thereby regulating gene expression .

  • Histone Modification: RBBP4/7 regulate histone deacetylation and methylation, key processes in chromatin remodeling and gene expression regulation . RBBP7 inhibits DNA methyltransferase 1, affecting DNA methylation and limiting transcription factor access to promoters .

  • Regulation of Cell Proliferation and Differentiation: RBBP7 interacts with the BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation .

  • Role in complexes: RBBP7 is an essential component of the histone acetyltransferase 1 (HAT1) complex, NuRD complex, Sin3A complex, and polycomb repressive complex 2 (PRC2) .

Interactions

RBBP7 interacts with several proteins :

  • BRCA1

  • GATAD2B

  • HDAC1

  • MTA2

  • Retinoblastoma protein

  • SAP30

  • SIN3A

  • p300

Role in Diseases

RBBP4/7 have a strong association with major human diseases :

  • Cancer: Upregulation of RBBP7 in bladder cancer results in a cell invasion phenotype, while a decrease in its expression inhibits cell invasion .

  • Age-related Memory Loss

  • Infectious Diseases

  • Alzheimer's Disease: RBBP7 may play a role in regulating p300-induced tau acetylation and subsequent tau pathogenesis in Alzheimer's disease and related tauopathies .

RBBP4/7 in Chromatin Remodeling

RBBP4/7 play multifaceted roles in chromatin remodeling :

ProcessDescription
DeacetylationRBBP4/7 interacts with the tails of histones H3 and H4, promoting the deacetylase activity of the HDAC complex, resulting in the removal of acetyl groups from H3/H4. This leads to chromatin compaction, preventing gene promoter transcription and inhibiting gene expression .
MethylationIn PRC2, RBBP4 recruits SUZ12 to PRC2 target sites. The EZH2 subunit serves as the methyltransferase active center, performing primary, secondary, and tertiary methylation on the 27th lysine of histone H3, resulting in H3K27me3 formation. EED binds to the H3K27me3 mark and further enhances the methyltransferase activity of EZH2. PRC2-mediated methylation induces chromatin compaction, limiting the binding of transcription factors and RNA polymerases, thus enabling gene silencing .
HAT1 complexRBBP7 is known to be an essential component of the histone acetyltransferase 1 (HAT1) complex .

Product Specs

Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Lead Time
Delivery times vary depending on the purchasing method and location. Please contact your local distributor for precise delivery estimates.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires advance notice and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our standard glycerol concentration is 50%, provided as a guideline.
Shelf Life
Shelf life depends on several factors, including storage conditions, buffer components, temperature, and the protein's inherent stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms maintain stability for 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt; aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing.
The tag type is determined during production. If you require a specific tag, please inform us; we will prioritize its development.
Synonyms
Rbbp7; Histone-binding protein RBBP7; Nucleosome-remodeling factor subunit RBAP46; Retinoblastoma-binding protein 7; RBBP-7
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
2-425
Protein Length
Full Length of Mature Protein
Purity
>85% (SDS-PAGE)
Species
Rattus norvegicus (Rat)
Target Names
Rbbp7
Target Protein Sequence
ASKEMFEDT VEERVINEEY KIWKKNTPFL YDLVMTHALQ WPSLTVQWLP EVTKPEGKDY ALHWLVLGTH TSDEQNHLVV ARVHIPNDDA QFDASHCDSD KGEFGGFGSV TGKIECEIKI NHEGEVNRAR YMPQNPHIIA TKTPSSDVLV FDYTKHPAKP DPSGECNPDL RLRGHQKEGY GLSWNSNLSG HLLSASDDHT VCLWDINAGP KEGKIVDAKA IFTGHSAVVE DVAWHLLHES LFGSVADDQK LMIWDTRSNT TSKPSHLVDA HTAEVNCLSF NPYSEFILAT GSADKTVALW DLRNLKLKLH TFESHKDEIF QVHWSPHNET ILASSGTDRR LNVWDLSKIG EEQSAEDAED GPPELLFIHG GHTAKISDFS WNPNEPWVIC SVSEDNIMQI WQMAENIYND EESDVTTSEL EGQGS
Uniprot No.
Q71UF4

Target Background

Function
RBBP7 is a core histone-binding subunit that potentially targets chromatin remodeling factors, histone acetyltransferases, and histone deacetylases to their histone substrates in a manner regulated by nucleosomal DNA. It is a component of several complexes regulating chromatin metabolism, including: the type B histone acetyltransferase (HAT) complex (essential for chromatin assembly post-DNA replication); the core histone deacetylase (HDAC) complex (promoting histone deacetylation and transcriptional repression); the nucleosome remodeling and histone deacetylase complex (NuRD complex) (mediating transcriptional repression through histone deacetylation and nucleosome remodeling); the PRC2/EED-EZH2 complex (involved in repressing homeotic genes during development); and the NURF (nucleosome remodeling factor) complex.
Gene References Into Functions
  1. Endogenous Mta1/2 forms a complex with chromodomain helicase (Chd)4, histone deacetylase (Hdac)1/2, RbAp46/48, and Mbd3 in the rat cerebellum. PMID: 24991957
Database Links

KEGG: rno:83712

STRING: 10116.ENSRNOP00000062148

UniGene: Rn.3600

Protein Families
WD repeat RBAP46/RBAP48/MSI1 family
Subcellular Location
Nucleus.

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