Recombinant Rat Nuclear protein MDM1 (Mdm1)
Description
Introduction to Recombinant Rat Nuclear Protein MDM1 (Mdm1)
Recombinant Rat Nuclear protein MDM1 (Mdm1) is a microtubule-binding protein that plays a crucial role in regulating centriole duplication and microtubule stability. It is primarily localized to the centrioles of dividing cells and differentiating multiciliated cells, suggesting its involvement in cell cycle regulation and cilia formation . This article will delve into the characteristics, functions, and research findings related to MDM1, providing a comprehensive overview of its significance in cellular biology.
Characteristics and Localization of MDM1
MDM1 is characterized by its ability to bind microtubules both in vivo and in vitro. It contains repeat motifs essential for efficient microtubule binding, similar to other microtubule-binding proteins like CCSAP . The localization of MDM1 is primarily at the centrioles, where it resides in the centriole lumen. This localization is critical for its function in regulating centriole duplication and maintaining microtubule stability .
Centriole Duplication Regulation
MDM1 acts as a negative regulator of centriole duplication. Overexpression of MDM1 leads to suppressed centriole duplication, while its depletion results in an increase in granular material indicative of early centriole formation intermediates . This regulatory function is crucial for maintaining proper centriole numbers and preventing aberrant cell division.
Microtubule Stability
MDM1 also influences microtubule stability. Its overexpression stabilizes microtubules, which can impact various cellular processes dependent on microtubule dynamics, such as cell migration and intracellular transport .
Research Findings and Implications
Recent studies have utilized advanced microscopy techniques, such as 3D-SIM, to precisely map MDM1's localization within centrioles. These studies have shown that MDM1 is closely associated with the centriole barrel, suggesting a direct interaction with microtubules within the centriole structure .
Experimental Models
Experiments involving MDM1 overexpression or depletion have provided insights into its role in centriole regulation. For instance, in human RPE-1 cells, MDM1 depletion leads to increased granular material around centrioles, indicating enhanced centriole formation .
Data Tables
While specific data tables related to recombinant rat nuclear protein MDM1 are not readily available, key findings can be summarized as follows:
| Feature | Description |
|---|---|
| Localization | Centrioles of dividing and multiciliated cells |
| Function | Negative regulator of centriole duplication, stabilizes microtubules |
| Binding | Microtubules through specific repeat motifs |
| Effect of Overexpression | Suppresses centriole duplication, stabilizes microtubules |
| Effect of Depletion | Increases granular material indicative of early centriole formation |
References
- https://pmc.ncbi.nlm.nih.gov/articles/PMC4626064/ - https://www.genecards.org/cgi-bin/carddisp.pl?gene=MDM1 - https://www.bosterbio.com/anti-mdm1-antibody-a09897-boster.html
Product Specs
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