Recombinant Rat Poly [ADP-ribose] polymerase 16 (Parp16)
Description
Introduction to Recombinant Rat Poly [ADP-ribose] Polymerase 16 (Parp16)
Recombinant Rat Poly [ADP-ribose] Polymerase 16 (Parp16) is a protein that belongs to the family of Poly [ADP-ribose] polymerases (PARPs), which are enzymes involved in various cellular processes, including DNA repair, transcriptional regulation, and cell death. Parp16, specifically, is known for its role in mono-ADP-ribosylation, a post-translational modification that affects protein function and signaling pathways.
Key Features of Recombinant Rat Parp16
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Species: Rat
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Source: Expressed in Escherichia coli (E. coli)
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Tag: N-terminal His tag
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Protein Length: Full length (1-322 amino acids)
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Form: Lyophilized powder
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Purity: Greater than 90% as determined by SDS-PAGE
Structure and Function
Parp16 is structurally characterized by its ability to catalyze the transfer of ADP-ribose units from NAD+ to target proteins, thereby modifying their activity. The recombinant form of Parp16 is designed to mimic the native protein's function, facilitating research into its roles in cellular processes.
Amino Acid Sequence
The amino acid sequence of recombinant rat Parp16 is crucial for understanding its structural and functional properties. The sequence begins with MQLSNRAAARESVSRDVLAADLRCSLFASALQSYKRDSVLRPFPASYARHDCKDFEALLADTGRLPNLKELLQSSRDTDKQTWDLVSWILSSKILTIHSAEKAEFEKIQQLTGAPHTPVP IPDFLFEIEYFDPANARFYETKGGRDLIYAFHGSRLENFHSIIHNGLHCHLNKTSLFGEG TYLTSDLSLALIYSPHGRGWQHSLLGQTLSCVAVCEVIDHPDVKCQTKKKDSKEIDRSRA RIKHSEGGDVPPKYFVVTNNQLLRVKYLLVYSQKQPKRASSQLSWLCSHWFMVAMSLYLL LLLIVSVTNSSAFQHFWNRLKR .
Research Applications
Recombinant rat Parp16 is used in various research applications, including studies on protein modification, cell signaling, and disease mechanisms. Its recombinant form allows for controlled experiments to understand the enzyme's activity and interactions.
Inhibition Studies
Inhibition studies involving Parp16 often focus on compounds that selectively target this enzyme. For instance, compounds like 6 have been identified as potent inhibitors of Parp16, with minimal activity against other PARP isoforms like Parp1 and Parp3 . The IC50 values for such inhibitors provide insight into their potency and specificity.
| Compound | PARP1 (μM) | PARP3 (μM) | PARP16 (μM) |
|---|---|---|---|
| 1 | 24.3 | 3.4 | 0.427 |
| 5 | 20.1 | 3.1 | 296.3 |
| 6 | 17.8 | 74.1 | 0.362 |
Product Specs
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The tag type is determined during production. If you require a specific tag, please inform us for preferential development.
Target Background
Recombinant Rat Poly [ADP-ribose] polymerase 16 (Parp16) is an intracellular mono-ADP-ribosyltransferase potentially involved in various cellular processes through the mono-ADP-ribosylation of target proteins. It may play a role in the unfolded protein response (UPR) by activating key UPR effectors EIF2AK3 and ERN1 via ADP-ribosylation. Additionally, it may mono-ADP-ribosylate the nuclear import factor karyopherin KPNB1. Unlike some other mono-ADP-ribosyltransferases, Parp16 may not modify proteins at arginine or cysteine residues.
