Recombinant Rat Prostaglandin D2 receptor (Ptgdr)
Description
Molecular Structure and Recombinant Production
Gene and Protein Characteristics
Ptgdr is encoded by the Ptgdr gene in rats, which shares structural homology with human PTGDR. The receptor consists of 359 amino acids (human homolog) and contains seven transmembrane domains typical of GPCRs . Recombinant Ptgdr is produced in various systems, including E. coli, mammalian cells (e.g., HEK293), and wheat germ .
Primary Functions
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Mast Cell Maturation: Ptgdr promotes mast cell maturation and degranulation via PLA2G3-dependent pathways .
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Inflammatory Response: Mediates PGD2-induced vasodilation and leukocyte recruitment .
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Neurological Roles: While not directly implicated in Alzheimer’s disease (AD), PGD2 signaling through DP2 receptors has been studied in AD models .
Signaling Mechanism
Ptgdr couples with G<sub>s</sub> proteins, activating adenylate cyclase to elevate intracellular cAMP. Calcium mobilization occurs without IP<sub>3</sub> production . This pathway contrasts with DP2 receptor signaling, which involves G<sub>i</sub> proteins and reduced cAMP .
Recombinant Applications in Research
Measurement and Analysis Tools
The Rat Prostaglandin D2 Receptor ELISA Kit (Assay Genie) enables precise quantification of Ptgdr in serum, plasma, and cell culture supernatants .
| Parameter | Specification |
|---|---|
| Detection Range | 0.78–50 ng/mL |
| Sensitivity | 0.33 ng/mL |
| Intra-Assay CV | 4.2% |
| Inter-Assay CV | 8.4% |
| Sample Types | Serum, plasma, tissue homogenates, cell culture supernates |
Use Cases
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Inflammatory Disease Research: Ptgdr is a biomarker for asthma-related traits and allergic responses .
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Functional Studies: Recombinant Ptgdr is used in binding assays to study PGD2 interactions .
Research Findings and Disease Associations
Asthma and Allergy
Genetic variations in Ptgdr are linked to asthma susceptibility (ASRT1) . Ptgdr’s role in mast cell activation underscores its therapeutic potential in allergic diseases .
Neurological Insights
While Ptgdr itself is not directly studied in Alzheimer’s, PGD2 levels in rat hippocampi are 14.5-fold higher than PGE2, suggesting broader PGD2 pathway involvement in neuroinflammation . DP2 receptor antagonists (e.g., timapiprant) reduce AD pathology in transgenic rats, highlighting PGD2’s dual roles in health and disease .
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them during order placement. We will accommodate your request if possible.
Note: All proteins are shipped with standard blue ice packs. If dry ice shipping is required, please inform us in advance. Additional fees may apply.
Generally, liquid form has a shelf life of 6 months at -20°C/-80°C. Lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Target Background
- The inhibitory effect of PGD(2) in the small intestine is mediated via activation of the DP1 receptor. PMID: 18212515
