Recombinant Rat Protein GPR108 (Gpr108)
Description
Introduction to Recombinant Rat Protein GPR108
Recombinant Rat Protein GPR108, also known as G protein-coupled receptor 108, is a member of the G protein-coupled receptor superfamily. It has been identified as a crucial entry factor for adeno-associated viruses (AAVs), which are widely used in gene therapy due to their safety profile and efficiency in delivering genetic material to cells. GPR108 plays a significant role in facilitating the entry of most AAV serotypes into cells, with the exception of AAV5, which does not require GPR108 for entry .
Structure and Function of GPR108
GPR108 is a seven-transmembrane receptor, typical of the GPCR family. It is highly conserved across species, including humans and rodents, which makes it an important target for studying AAV tropism and developing gene therapy models . The receptor's N- and C-terminal domains are essential for its function in AAV entry .
Key Features of GPR108:
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Family: G protein-coupled receptor superfamily
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Function: AAV entry factor, involved in immune modulation and NF-κB signaling
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Species Conservation: Highly conserved between humans and rodents
Role in Adeno-Associated Virus (AAV) Entry
GPR108 is critical for the efficient entry of AAVs into cells. It acts alongside another entry factor, AAVR, to facilitate the transduction process. The absence of GPR108 significantly reduces the ability of most AAV serotypes to enter cells, except for AAV5, which is GPR108-independent .
AAV Serotypes and GPR108 Dependency:
| AAV Serotype | GPR108 Dependency |
|---|---|
| AAV5 | Independent |
| AAV8 | Dependent |
| AAVrh32.33 | Dependent |
Research Findings and Applications
Research on GPR108 has provided valuable insights into the mechanisms of AAV entry and has implications for gene therapy. The identification of GPR108 as a conserved entry factor across species supports its potential use in developing more effective gene therapy vectors .
Applications of GPR108 Research:
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Gene Therapy: Understanding GPR108's role in AAV entry can improve vector design and targeting efficiency.
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Basic Research: Insights into GPR108's function contribute to understanding cellular immune responses and signaling pathways.
Recombinant Protein Production and Use
While specific information on recombinant rat GPR108 protein production is limited, recombinant proteins are generally produced using expression systems like E. coli or mammalian cells. These proteins are used in various applications, including Western blotting, immunohistochemistry, and functional assays to study protein function and interactions .
Recombinant Protein Applications:
| Application | Description |
|---|---|
| Western Blot | Detects protein expression levels |
| Immunohistochemistry | Localizes protein in tissues |
| Functional Assays | Studies protein activity and interactions |
Product Specs
Note: While we will prioritize shipping the format currently in stock, we are happy to accommodate specific format requests. Please indicate your preference in the order notes section, and we will prepare accordingly.
Note: All proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance, as additional charges will apply.
Generally, the shelf life of liquid protein is 6 months at -20°C/-80°C. Lyophilized protein typically has a shelf life of 12 months at -20°C/-80°C.
If you require a specific tag type, please inform us, and we will prioritize its development.
