Recombinant Rat Transmembrane protein 176A (Tmem176a)
Description
Production and Purification
Recombinant Rat TMEM176A is synthesized using mammalian expression systems (e.g., HEK293 cells) or E. coli . Key production parameters include:
Custom production options are available for specific experimental needs, with lead times of 5–9 weeks .
Research Applications
Recombinant Rat TMEM176A is utilized in:
-
Mechanistic Studies: Investigating its role in ion channel regulation, dendritic cell differentiation, and immune responses .
-
Disease Models: Linked to hepatocellular carcinoma (via HCA112 alias) and long QT syndrome .
-
Drug Interaction Screens: Used to assess transcriptional responses to toxins, pharmaceuticals, and environmental agents .
-
Protein Interaction Mapping: Identified interactors include HIVEP1, TGM2, and HRSP12, suggesting roles in transcriptional regulation and signal transduction .
Modulation of TMEM176A Expression
Studies using recombinant TMEM176A have revealed its sensitivity to diverse compounds:
| Compound | Effect on TMEM176A Expression | Study Model | Source |
|---|---|---|---|
| Clofibrate | Decreased | Rat hepatocytes | |
| Asbestos | Increased | Mouse lung tissue | |
| Cyclosporin A | Decreased | Human cell lines | |
| Rotenone | Increased | Rat neuronal cells |
These findings highlight its regulatory role in toxicological and pharmacological responses .
Functional Insights
-
Immune Regulation: TMEM176A negatively regulates dendritic cell differentiation, impacting antigen presentation .
-
Environmental Stress Response: Upregulated by titanium dioxide and downregulated by perfluorinated compounds .
Challenges and Future Directions
While recombinant TMEM176A has advanced in vitro studies, challenges persist:
-
Functional Complexity: Its exact biochemical role (e.g., ion channel vs. signaling scaffold) remains unresolved .
-
Species-Specific Variants: Cross-reactivity differences between rat, human, and mouse homologs necessitate cautious interpretation .
Ongoing research aims to elucidate its therapeutic potential in autoimmune diseases and cancer .
Product Specs
Note: While we will prioritize shipping the format currently in stock, we understand your specific needs. Please indicate any format preferences when placing your order, and we will fulfill your request whenever possible.
Note: All our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please notify us in advance as additional fees will apply.
Generally, liquid forms have a shelf life of 6 months at -20°C/-80°C. Lyophilized forms have a shelf life of 12 months at -20°C/-80°C.
Target Background
KEGG: rno:297077
UniGene: Rn.17071
