Recombinant Rhizobium leguminosarum bv. viciae Undecaprenyl-diphosphatase (uppP)
Description
Enzymatic Function and Biological Role
UppP (EC 3.6.1.27) performs the reaction:
This activity is enhanced by divalent cations like Ca²⁺ . In Rhizobium leguminosarum, UppP contributes to:
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Peptidoglycan biosynthesis: Recycling undecaprenyl phosphate for cell wall assembly .
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Bacitracin resistance: Bacitracin binds undecaprenyl diphosphate, and UppP activity counteracts this inhibition .
Applications in Research
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Antibiotic Resistance Studies: UppP’s role in bacitracin resistance makes it a target for antimicrobial strategies .
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Enzyme Kinetics: Used to study divalent cation dependence and substrate specificity .
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Symbiotic Function: R. leguminosarum UppP may influence nodulation efficiency in legumes by modulating cell envelope integrity .
Key Research Findings
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Plasmid Rearrangement: In R. leguminosarum bv. viciae VF39, symbiotic plasmid (pSym) rearrangements alter uppP localization, affecting bacterial adaptability .
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Transcriptional Regulation: Deletion of plasmid pRleVF39b in R. leguminosarum increases uppP expression by 4-fold, suggesting plasmid-mediated repression .
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EPS Biosynthesis Link: UppP activity indirectly affects exopolysaccharide (EPS) production via UDP-glucose pool modulation .
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please specify them when placing your order, and we will accommodate your request.
Note: All proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance. Additional fees may apply.
Generally, liquid form has a shelf life of 6 months at -20°C/-80°C. Lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Target Background
KEGG: rle:RL0328
STRING: 216596.RL0328
