Recombinant Rhizobium loti ATP synthase subunit b 1 (atpF1)
Description
Introduction to Recombinant Rhizobium loti ATP Synthase Subunit b 1 (atpF1)
Recombinant Rhizobium loti ATP synthase subunit b 1, also known as atpF1, is a protein component of the ATP synthase complex in Rhizobium loti, a bacterium that forms symbiotic relationships with leguminous plants. This subunit is part of the F0 sector of the ATP synthase, which plays a crucial role in energy production by facilitating the transport of protons across the membrane to drive ATP synthesis.
Structure and Function
The ATP synthase complex consists of two main parts: F1 and F0. The F1 portion is responsible for the catalytic activity of ATP synthesis, while the F0 sector, where the subunit b 1 is located, acts as a proton channel. The subunit b 1 in Rhizobium loti is involved in the assembly and function of the F0 sector, ensuring efficient proton translocation and ATP production.
| Subunit | Function | Location |
|---|---|---|
| b 1 (atpF1) | Proton channel component | F0 sector |
Recombinant Production
Recombinant Rhizobium loti ATP synthase subunit b 1 is produced through genetic engineering techniques. This involves cloning the gene encoding the subunit into an expression vector, which is then introduced into a host organism for protein production. The recombinant protein is often used in research and diagnostic applications, such as ELISA assays, to study its structure, function, and interactions with other components of the ATP synthase complex.
| Product Details | Description |
|---|---|
| Species | Rhizobium loti (strain MAFF303099) |
| Quantity | 50 µg (other quantities available upon request) |
| Storage Buffer | Tris-based buffer, 50% glycerol |
| Storage Conditions | -20°C or -80°C for extended storage |
Applications and Future Directions
The recombinant production of ATP synthase subunits like atpF1 from Rhizobium loti can facilitate detailed biochemical and structural studies. These studies can provide insights into the mechanisms of ATP synthesis and proton translocation, potentially leading to the development of novel antimicrobial agents or bioenergetic tools.
Product Specs
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Target Background
F1F0 ATP synthase synthesizes ATP from ADP utilizing a proton or sodium gradient. This enzyme comprises two domains: the extramembranous catalytic F1 domain and the membrane-embedded proton channel F0 domain, connected by a central and peripheral stalk. ATP synthesis within the F1 catalytic domain is coupled to proton translocation via a rotary mechanism involving the central stalk subunits.
This protein is a component of the F0 channel, forming part of the peripheral stalk which links F1 and F0.
KEGG: mlo:mlr7413
STRING: 266835.mlr7413
